INQUIMAE, Facultad de Ciencias Exactas y Naturales, CONICET - Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina.
IQUIBICEN, Facultad de Ciencias Exactas y Naturales, CONICET - Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina.
Bull Environ Contam Toxicol. 2020 Dec;105(6):835-840. doi: 10.1007/s00128-020-03031-4. Epub 2020 Nov 7.
Gills represent one of the major sites of gas exchange of fish, consequently they are in continuous close contact with the aquatic environment and its pollutants. In the present study the effects on gills of pejerrey fish, Odontesthes bonariensis, under glyphosate-based herbicide subchronic exposure were analyzed. Adult animals were exposed to sublethal concentrations of a glyphosate-based commercial formulation (1 and 10 PMG mg L, PMG: glyphosate active ingredient) for 15 days, while control group was maintained in rearing water. Ultrastructural changes in gills were observed by scanning electron microscopy (SEM). The composition of the surface epithelium and specific surface area were determined by energy dispersive spectroscopy (EDS) and N (g) adsorption-desorption isotherms, respectively. The herbicide exposure induced severe alterations in gill ultrastructure, as shown in the SEM micrographs. Accordingly, an increase in surface area of the gills of exposed animals was determined. These results support that gills parameters of freshwater fish are sensitive morphological biomarkers for glyphosate exposure.
鳃是鱼类气体交换的主要部位之一，因此它们与水生态环境及其污染物持续密切接触。本研究分析了受草甘膦基除草剂亚慢性暴露影响的巴塔戈尼亚牙鱼（Odontesthes bonariensis）鳃的变化。成年鱼被暴露在草甘膦基商业制剂的亚致死浓度下（1 和 10 PMG mg/L，PMG：草甘膦有效成分）15 天，而对照组则在养殖水中维持。通过扫描电子显微镜（SEM）观察鳃的超微结构变化。通过能量色散光谱（EDS）和 N（g）吸附-解吸等温线分别确定表面上皮的组成和比表面积。SEM 显微照片显示，除草剂暴露会严重改变鳃的超微结构。因此，确定了暴露动物的鳃表面积增加。这些结果表明，淡水鱼类的鳃参数是对草甘膦暴露的敏感形态生物标志物。
