Laser Therapy Centre, Department of Surgical and Diagnostic Sciences (D.I.S.C.), University of Genova, Genova, Italy.
Department of Orthopaedic Dentistry, Sechenov First Moscow State Medical University, Trubetzkaya St., 8, Bd. 2, 119991, Moscow, Russian Federation.
Cell Tissue Res. 2021 Mar;383(3):1003-1016. doi: 10.1007/s00441-020-03306-6. Epub 2020 Nov 7.
Tailoring the cell organelles and thus changing cell homeostatic behavior has permitted the discovery of fascinating metabolic features enabling enhanced viability, differentiation, or quenching inflammation. Recently, photobiomodulation (PBM) has been accredited as an effective cell manipulation technique with promising therapeutic potential. In this prospective, in vitro results revealed that 808-nm laser light emitted by a hand-piece with a flat-top profile at an irradiation set up of 60 J/cm2 (1 W, 1 W/cm; 60 s, continuous wave) regulates bone marrow stromal cell (BMSC) differentiation toward osteogenesis. Considering the importance of actin cytoskeleton reorganization, which controls a range of cell metabolic activities, comprising shape change, proliferation and differentiation, the aim of the current work is to assess whether PBM therapy, using a flat-top hand-piece at higher-fluence irradiation on BMSCs, is able to switch photon signals into the stimulation of biochemical/differentiating pathways involving key activators that regulate de novo actin polymerization. Namely, for the first time, we unearthed the role of the flat-top hand-piece at higher-fluence irradiation on cytoskeletal characteristics of BMSCs. These novel findings meet the needs of novel therapeutically protocols provided by laser treatment and the manipulation of BMSCs as anti-inflammatory, osteo-inductive platforms.
定制细胞细胞器，从而改变细胞内稳态行为，可以发现迷人的代谢特征，从而提高细胞活力、分化或抑制炎症。最近，光生物调节（PBM）已被认为是一种有效的细胞操作技术，具有有前途的治疗潜力。在这项前瞻性、体外研究结果表明，在 60J/cm2（1W、1W/cm；60s，连续波）辐照设置下，由具有平顶轮廓的手持件发射的 808nm 激光可调节骨髓基质细胞（BMSC）向成骨分化。考虑到细胞代谢活动的范围包括形状变化、增殖和分化，细胞骨架重组的重要性，目前工作的目的是评估使用平顶手持件在高剂量辐照下对 BMSC 的 PBM 治疗是否能够将光子信号转换为刺激涉及调节新形成的肌动蛋白聚合的关键激活剂的生化/分化途径。也就是说，我们首次揭示了平顶手持件在高剂量辐照下对 BMSC 细胞骨架特征的作用。这些新发现满足了激光治疗和操纵 BMSC 作为抗炎、成骨诱导平台提供的新型治疗方案的需求。
