Ravera Silvia, Bertola Nadia, Pasquale Claudio, Bruno Silvia, Benedicenti Stefano, Ferrando Sara, Zekiy Angelina, Arany Praveen, Amaroli Andrea
Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy.
Department of Surgical and Diagnostic Sciences, University of Genoa, 16132 Genoa, Italy.
Biomedicines. 2021 Nov 18;9(11):1717. doi: 10.3390/biomedicines9111717.
Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence.
光生物调节疗法（PBM）是一种低剂量光疗形式，通过非电离源传递能量起作用。在最近二十年里，PBM在医学领域的接受度取得了巨大进展。然而，PBM对现有恶性或癌前细胞潜在刺激的影响仍不清楚。因此，主要终点是评估PBM治疗参数对头颈部鳞状细胞癌（HNSCC）增殖或存活的安全性。次要终点是评估PBM治疗的任何假定抗癌作用。在人头颈部鳞状细胞癌细胞模型（OHSU-974 FAcorr细胞系）上研究了细胞活力、能量代谢、氧化应激以及促凋亡和抗凋亡标志物的表达。PBM治疗通过810nm二极管激光（GaAlAs）设备（Garda Laser，意大利维罗纳内格拉尔7024），以连续波（CW）模式，在功率为0、0.25、0.50、0.75、1.00或1.25W的条件下进行，曝光时间为60秒，光斑尺寸为1cm，与细胞的距离为1.86cm。结果表明，810nm的PBM影响了OHSU-971 FAcorr中的氧化磷酸化，导致代谢转换为无氧糖酵解。此外，PBM降低了过氧化氢酶活性，导致氧化应激产生与抗氧化防御之间失衡，这可能刺激细胞促凋亡途径。在研究的参数下，我们的数据表明PBM作为一种支持性癌症治疗方法是安全的。需要进行临床前和临床研究来证实体外证据。
