Harrison Alisha M, Soby Scott D
Biomedical Sciences Program, College of Graduate Studies, Midwestern University, 19555 N 59th Avenue, Glendale, AZ, 85308, USA.
College of Veterinary Medicine, Midwestern University, 19555 N 59th Avenue, Glendale, AZ, 85308, USA.
AMB Express. 2020 Nov 7;10(1):202. doi: 10.1186/s13568-020-01140-1.
The precipitous drop in the cost of genomic sequencing and the concomitant availability of computational methods for comparing genome-level data has made the accurate taxonomic placement of bacteria affordable and relatively rapid. Inaccurate taxonomic placement of bacteria has serious implications in clinical, environmental, and regulatory microbiology, but it can also adversely affect interpretation of research results. The quorum biosensor strain CV026 was derived from an isolate of Chromobacterium that was labeled as C. violaceum ATCC 31532, and is catalogued by the ATCC under that species name. Nearly 200 papers have been published that use CV026 as an indicator for quorum sensing activity in many Gram negative bacteria, but the inability of C. violaceum strains to complement the quorum sensing mutation in CV026 has called the taxonomic placement of the parent strain into question. We used molecular phylogeny and a large number of metabolic and phenotypic characters to demonstrate that Chromobacterium strain ATCC 31532 is a member of species Chromobacterium subtsugae.
基因组测序成本的急剧下降以及随之而来的用于比较基因组水平数据的计算方法的出现，使得细菌的准确分类定位变得经济且相对快速。细菌分类定位不准确在临床、环境和监管微生物学中具有严重影响，但也会对研究结果的解释产生不利影响。群体感应生物传感器菌株CV026源自一株被标记为紫色色杆菌ATCC 31532的色杆菌分离株，并由美国典型培养物保藏中心以该物种名称编入目录。已有近200篇论文发表，使用CV026作为许多革兰氏阴性菌群体感应活性的指标，但紫色色杆菌菌株无法互补CV026中的群体感应突变，这使得亲本菌株的分类定位受到质疑。我们利用分子系统发育以及大量的代谢和表型特征来证明，色杆菌菌株ATCC 31532是Subtsugae色杆菌物种的一个成员。
