基于罗丹明 B/氨基酸衍生物的“开启”型荧光探针用于水中 Fe 的检测。
"Turn-on" fluorescent probes based on Rhodamine B/amino acid derivatives for detection of Fe in water.
机构信息
Department of Forensic Chemistry, Criminal Investigation Police University of China, Shenyang 110035, China.
College of Chemistry, Jilin Normal University, Siping 136000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119095. doi: 10.1016/j.saa.2020.119095. Epub 2020 Oct 24.
Five kinds of Fe fluorescent probes (RhB-Gly, RhB-Ala, RhB-Try, RhB-Cys, and RhB-His) were synthesized and characterized by NMR and mass spectrometry, based on the "OFF-ON" mechanism of Rhodamine B derivatives. The RhB-His based probe showed remarkable sensing performance toward the detection for Fe and showed high selectivity for Fe in the presence of other metal ions (such as Fe, Hg, Zn, Ba, Al, Co, Cd, K, Na, Mn, Pd, Pb, Ca, Ni, Cu, and Ag), in PBS buffer solution (containing 2% of EtOH, pH 7.4, 1.0 mmol/L). The enhancement of the fluorescence was linearly proportional with the concentration Fe (from 0 to 20 μmol/L), while the detection limit reached 0.88 μmol/L with a response time of 15 s. The RhB-His probe was successfully applied to investigate real samples and living cell imaging.
基于罗丹明 B 衍生物的“关-开”机制，我们合成并通过核磁和质谱对 5 种 Fe 荧光探针（RhB-Gly、RhB-Ala、RhB-Try、RhB-Cys 和 RhB-His）进行了表征。基于 RhB-His 的探针对 Fe 的检测表现出显著的感应性能，并在存在其他金属离子（如 Fe、Hg、Zn、Ba、Al、Co、Cd、K、Na、Mn、Pd、Pb、Ca、Ni、Cu 和 Ag）时，在 PBS 缓冲液（含 2% EtOH，pH 7.4，1.0 mmol/L）中对 Fe 具有高选择性。荧光的增强与 Fe 的浓度呈线性比例（从 0 到 20 μmol/L），而检测限达到 0.88 μmol/L，响应时间为 15 s。该 RhB-His 探针成功应用于实际样品和活细胞成像的研究。
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