Reinsmoen N L, Bach F H
Immunobiology Research Center, University of Minnesota Hospital and Clinic, Minneapolis 55455.
Hum Immunol. 1987 Sep;20(1):13-31. doi: 10.1016/0198-8859(87)90003-6.
Individuals carrying the serologically-defined DR2 haplotypes can be further subdivided by utilizing T cell response to define several HLA-Dw/LD clusters: Dw2, Dw12, LD-5a, and a related group composed of FJO, AZH, and MN2. We have used cytotoxic T cell clones generated against these DR2 haplotypes to define the DR and DQ associated Dw/LD polymorphisms. Clones were categorized as DR, DQ, DP, or class I-directed based on monoclonal antibody (MoAb) inhibition studies. We analyzed the distribution of the determinants detected by these clones based on analysis with panels of cells. Some DR-directed clones only lysed cells positive for the Dw subtype of the sensitizing cell. A few DR-directed clones lysed most DR2 positive cells. Other DR-directed clones detected determinants shared by cells of different subtypes: determinants shared by Dw2 and Dw12 positive cells and determinants shared by MN2, FJO, AZH, and LD-5a positive cells. The majority of the DQ-directed clones only lysed cells positive for the sensitizing HLA-Dw subtype. A group of clones primed against an LD-5a HTC, whose cytolytic activity was not blocked by any monoclonal antibodies tested, lysed some, but not all, DR2 positive cells as well as Dw1 and Dw10 positive cells. The results suggest the detection of determinants on these molecules which may be shared by cells of specific Dw subgroups and determinants shared by most cells of the sensitizing serological specificity. The basis of this observed polymorphism and influence on the allogeneic response are discussed.
携带血清学定义的DR2单倍型的个体可通过利用T细胞反应进一步细分，以定义几个HLA-Dw/LD簇：Dw2、Dw12、LD-5a以及由FJO、AZH和MN2组成的相关组。我们使用针对这些DR2单倍型产生的细胞毒性T细胞克隆来定义与DR和DQ相关的Dw/LD多态性。根据单克隆抗体（MoAb）抑制研究，克隆被分类为DR、DQ、DP或I类定向。我们基于细胞系分析了这些克隆检测到的决定簇的分布。一些DR定向克隆仅裂解对致敏细胞的Dw亚型呈阳性的细胞。少数DR定向克隆裂解大多数DR2阳性细胞。其他DR定向克隆检测到不同亚型细胞共有的决定簇：Dw2和Dw12阳性细胞共有的决定簇以及MN2、FJO、AZH和LD-5a阳性细胞共有的决定簇。大多数DQ定向克隆仅裂解对致敏HLA-Dw亚型呈阳性的细胞。一组针对LD-5a HTC致敏的克隆，其细胞溶解活性未被任何测试的单克隆抗体阻断，裂解了一些但不是所有的DR2阳性细胞以及Dw1和Dw10阳性细胞。结果表明在这些分子上检测到了可能由特定Dw亚组的细胞共有的决定簇以及由致敏血清学特异性的大多数细胞共有的决定簇。讨论了这种观察到的多态性的基础及其对同种异体反应的影响。
