Reinsmoen N L, Layrisse Z, Betuel H, Freidel A C, Bach F H
Hum Immunol. 1984 Oct;11(2):105-15. doi: 10.1016/0198-8859(84)90049-1.
HLA-Dw2 and Dw12 are both associated with HLA-DR2; however, these specificities accounts for only 86% (161/188) of the DR2+ haplotypes in our North American Caucasian panel. In an attempt to identify new DR2 associated antigenic clusters, we have generated four primed lymphocyte (LD) typing (PLT) reagents in haploidentical familial combination against DR2+ Dw blank haplotypes. These reagents were positively restimulated by 11 of 16 DR2+ Dw blank cells tested, with good discrimination from Dw2 and Dw12+ cells, thus identifying a new antigenic cluster provisionally termed LD-MN2. We have compared the LD-MN2 specificity with the specificity LD-5a defined by two DR2+ HTCs, BAS and REM, (Layrisse, Caracas) which have been included in the pre-1984 Workshop Cluster DB9. Although none of our DR2+ cells gave typing responses to these two HTCs defining LD-5a, PLT studies did indicate an interrelationship between these specificities and with the specificity tb24 defined with the HTC, FJO (Betuel). The LD-5a HTCs, four LD-5a heterozygous cells, and two additional HTCs (WJR-Hansen, Seattle and FJO/tb24--Betuel, Lyon) significantly restimulated the anti-MN2 PLT reagents, though usually not as strongly as the MN2+ cells. MN2+ cells primed against the LD-5a HTCs were restimulated by only the LD-5a+ cells. Dw2+ cells primed against FJO were restimulated by some, but not all MN2+ cells. These results suggest that MN2, tb24, and LD-5a share some determinants, not shared with most cells which type as Dw2 and Dw12, though differing by other stimulatory determinants. These studies emphasize the necessity of studying new antigenic clusters by both PLT and HTC methodologies as well as testing different ethnic groups.
HLA - Dw2和Dw12均与HLA - DR2相关；然而，在我们的北美白种人样本中，这些特异性仅占DR2 +单倍型的86%（161/188）。为了识别新的与DR2相关的抗原簇，我们针对DR2 + Dw空白单倍型，以单倍型相同的家族组合方式产生了四种引发淋巴细胞（LD）分型（PLT）试剂。所测试的16个DR2 + Dw空白细胞中有11个对这些试剂产生了阳性再刺激反应，与Dw2和Dw12 +细胞有良好的区分度，从而识别出一个新的抗原簇，暂称为LD - MN2。我们已将LD - MN2特异性与由两个DR2 + HTC（BAS和REM，位于加拉加斯的拉伊里斯）定义的特异性LD - 5a进行了比较，这两个HTC已被纳入1984年前的研讨会聚类数据库DB9。尽管我们的DR2 +细胞对定义LD - 5a的这两个HTC均未给出分型反应，但PLT研究确实表明这些特异性之间以及与由HTC FJO（位于里昂的贝图埃尔）定义的特异性tb24之间存在相互关系。LD - 5a HTC、四个LD - 5a杂合细胞以及另外两个HTC（位于西雅图的WJR - 汉森和位于里昂的FJO/tb24 - 贝图埃尔）显著再刺激了抗MN2 PLT试剂，不过通常不如MN2 +细胞强烈。针对LD - 5a HTC引发的MN2 +细胞仅被LD - 5a +细胞再刺激。针对FJO引发的Dw2 +细胞被一些但并非所有的MN2 +细胞再刺激。这些结果表明，MN2、tb24和LD - 5a共享一些决定簇，这些决定簇与大多数分型为Dw2和Dw12的细胞所共有的决定簇不同，尽管在其他刺激决定簇方面存在差异。这些研究强调了通过PLT和HTC方法以及测试不同种族群体来研究新抗原簇的必要性。
