Research Center for Analytical Sciences, Northeastern University, Shenyang, 110819, PR China.
Research Center for Analytical Sciences, Northeastern University, Shenyang, 110819, PR China.
Anal Chim Acta. 2020 Nov 22;1138:1-8. doi: 10.1016/j.aca.2020.09.010. Epub 2020 Sep 9.
Acid phosphatase is widely used as a clinical indicator because of its close correlation with a variety of diseases. Herein, a label-free and colorimetric sensing method for detecting the activity of acid phosphatase was constructed based on hollow mesoporous manganese dioxide nanospheres. The nanospheres exhibit superior oxidase-like property and can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to yellow TMB. Ascorbic acid from acid phosphatase-catalyzed hydrolysis of L-ascorbic acid-2-phosphate will inhibit the oxidization reaction, igniting vivid color variation. On the basis of this obvious multicolor change, the visual detection of acid phosphatase was achieved. Compared with the single-color change, the multicolor colorimetric method is more conducive for naked-eye discrimination. The absorbance difference at 450 nm exhibits a linear relationship with the concentration of acid phosphatase ranging from 1.0 to 25 U L, with a detection limit as low as 0.45 U L. Acid phosphatase in human serum samples was successfully determined. Moreover, the inhibition efficiency of NaF for acid phosphatase activity was investigated, proving the proposed colorimetric method will be a potential platform for screening acid phosphatase inhibitors and discovering new drugs.
酸性磷酸酶由于与多种疾病密切相关，因此被广泛用作临床指标。在此，基于中空介孔二氧化锰纳米球构建了一种用于检测酸性磷酸酶活性的无标记和比色传感方法。纳米球具有优异的过氧化物酶样性质，可以将无色的 3,3',5,5'-四甲基联苯胺（TMB）氧化为黄色的 TMB。酸性磷酸酶催化 L-抗坏血酸-2-磷酸水解产生的抗坏血酸会抑制氧化反应，引发生动的颜色变化。基于这种明显的多色变化，实现了对酸性磷酸酶的可视化检测。与单一颜色变化相比，多色比色法更有利于肉眼识别。在 450nm 处的吸光度差异与酸性磷酸酶浓度在 1.0 到 25 U L 范围内呈线性关系，检测限低至 0.45 U L。成功测定了人血清样品中的酸性磷酸酶。此外，还研究了 NaF 对酸性磷酸酶活性的抑制效率，证明了所提出的比色法将成为筛选酸性磷酸酶抑制剂和发现新药的潜在平台。
