Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada (LHYEDEC), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina; Facultad de Ciencias Veterinarias, Consejo nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Laboratorio de Patología Especial Veterinaria Dr. Bernardo Epstein, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Res Vet Sci. 2021 Mar;135:366-370. doi: 10.1016/j.rvsc.2020.10.018. Epub 2020 Oct 22.
Cutaneous papillomas (CPs) and cutaneous squamous cell carcinomas (CSCCs) are usual epidermal tumours in dogs. CPs and CSCCs probably arise from the neoplastic transformation of the keratinocytes within the stem cell compartment, since these cells are the only keratinocytes that would reside long enough to accumulate the number of molecular alterations to drive the progression towards a tumour cell phenotype. However, the role of these cells in common epidermal tumours in dogs is still unknown. Thus, the purpose of this study was to evaluate the immunohistochemical expression pattern of p63 together with CK5, molecular markers of epidermal stem cells, on sections of tissue microarrays constructed from canine samples of CP and CSCC to investigate the contribution of stem cells in those canine tumours. p63/CK5 coexpression was retained in most basal and some suprabasal cells in CPs and CSCCs. In addition, increased coexpression of these molecules was observed in a group of CPs and CSCCs, as a result of a higher p63 expression. These results suggest that the coexpression of p63/CK5 may mark epidermal keratinocytes that possess self-renewal capacity rather than only stem cells, and suggest that transit amplifying cells, and even differentiated keratinocytes, may also contribute to the pathogenesis of epidermal tumours in dogs.
皮肤乳头瘤（CPs）和皮肤鳞状细胞癌（CSCCs）是犬常见的表皮肿瘤。CPs 和 CSCCs 可能起源于干细胞区室中角质形成细胞的肿瘤性转化，因为这些细胞是唯一能够存活足够长时间以积累大量分子改变的角质形成细胞，从而推动向肿瘤细胞表型的进展。然而，这些细胞在犬常见的表皮肿瘤中的作用仍不清楚。因此，本研究的目的是评估 p63 与 CK5（表皮干细胞的分子标志物）在犬 CP 和 CSCC 组织微阵列切片中的免疫组织化学表达模式，以研究干细胞在这些犬肿瘤中的作用。CP 和 CSCC 中的大多数基底细胞和一些基底上层细胞中保留了 p63/CK5 的共表达。此外，由于 p63 表达增加，观察到一组 CP 和 CSCC 中这些分子的共表达增加。这些结果表明，p63/CK5 的共表达可能标记具有自我更新能力的表皮角质形成细胞，而不仅仅是干细胞，并表明过渡扩增细胞甚至分化的角质形成细胞也可能有助于犬表皮肿瘤的发病机制。
