Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences/Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Harbin), Ministry of Agriculture and Rural Areas, Harbin, P. R. China.
Department of Food Science and Engineering, College of Food Science and Technology, Shanghai Ocean University, Shanghai, P. R. China.
J Sep Sci. 2021 Jan;44(2):497-512. doi: 10.1002/jssc.202000861. Epub 2020 Nov 27.
It is critical to investigate the adaptive development and the physiological mechanism of fish in external stimulation. In this study, the response of Barbus capito to salinity-alkalinity exposure was explored by high-throughput nontargeted and liquid chromatography-mass spectrometry-based metabolomics to investigate metabolic biomarker and pathway changes. Meanwhile, the biochemical indexes of Barbus capito were measured to discover the chronic impairment response to salinity-alkalinity exposures. A total of 29 tissue metabolites were determined to deciphering the endogenous metabolic changes of fishes during the different concentration salinity-alkalinity exposures environment, which were mainly involved in the key metabolism including the phenylalanine, tyrosine, and tryptophan biosynthesis, arachidonic acid metabolism, pyruvate metabolism, citrate cycle, and glycerophospholipid metabolism. Finally, we found the amino acid metabolism as key target was associated with the endogenous metabolites and metabolic pathways of Barbus capito to salinity-alkalinity exposures. In conclusion, metabolomics is a potentially powerful tool to reveal the mechanism information of fish in various exposure environments.
研究鱼类在外界刺激下的适应发展和生理机制至关重要。本研究采用高通量非靶向和基于液相色谱-质谱联用的代谢组学技术，探索了高原鳅（Barbus capito）对盐度-碱度暴露的响应，以研究代谢生物标志物和途径的变化。同时，测量了高原鳅的生化指标，以发现其对盐度-碱度暴露的慢性损伤反应。共测定了 29 种组织代谢物，以揭示鱼类在不同浓度盐度-碱度暴露环境下的内源性代谢变化，这些变化主要涉及关键代谢途径，包括苯丙氨酸、酪氨酸和色氨酸生物合成、花生四烯酸代谢、丙酮酸代谢、柠檬酸循环和甘油磷脂代谢。最后，我们发现氨基酸代谢作为关键靶点与高原鳅盐度-碱度暴露的内源性代谢物和代谢途径有关。总之，代谢组学是一种潜在的强大工具，可以揭示鱼类在各种暴露环境下的机制信息。
