State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin 300072, PR China.
Adv Colloid Interface Sci. 2020 Nov;285:102286. doi: 10.1016/j.cis.2020.102286. Epub 2020 Oct 13.
Green fluorescence proteins (GFP) are appealing to a variety of biomedical and biotechnology applications, such as protein fusion, subcellular localizations, cell visualization, protein-protein interaction, and genetically encoded sensors. To mimic the fluorescence of GFP, various compounds, such as GFP chromophores analogs, hydrogen bond-rich proteins, and aromatic peptidyl nanostructures that preclude free rotation of the aryl-alkene bond, have been developed to adapt them for a fantastic range of applications. Herein, we firstly summarize the structure and luminescent mechanism of GFP. Based on this, the design strategy, fluorescent properties, and the advanced applications of GFP-inspired fluorophores are then carefully discussed. The diverse advantages of bioinspired fluorophores, such as biocompatibility, structural simplicity, and capacity to form a variety of functional nanostructures, endow them potential candidates as the next-generation bio-organic optical materials.
绿色荧光蛋白（GFP）在生物医学和生物技术的各种应用中很有吸引力，例如蛋白质融合、亚细胞定位、细胞可视化、蛋白质-蛋白质相互作用和遗传编码传感器。为了模拟 GFP 的荧光，已经开发了各种化合物，例如 GFP 发色团类似物、氢键丰富的蛋白质和芳香肽纳米结构，以阻止芳基-烯烃键的自由旋转，从而使它们适应各种应用。在此，我们首先总结 GFP 的结构和发光机制。在此基础上，然后仔细讨论了 GFP 启发的荧光团的设计策略、荧光特性和先进应用。生物启发荧光团具有生物相容性、结构简单和能够形成各种功能纳米结构的能力等多种优势，使它们成为下一代生物有机光学材料的潜在候选材料。
