Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 12, Brno, Czech Republic.
Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic.
Genomics. 2021 Jan;113(1 Pt 2):1109-1119. doi: 10.1016/j.ygeno.2020.11.007. Epub 2020 Nov 7.
Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium. By cultivation analysis we highlighted its specific behavior in comparison to two selected closely related strains. Despite the fact that several CRISPR loci were found, 16 putative prophages showed the ability to receive foreign DNA. Thus, the strain has the necessary features for future engineering of its 1,3-propanediol biosynthetic pathway and for the possible industrial utilization in the production of biofuels.
戴奥里氏梭菌（Clostridium diolis）DSM 15410 是一种能够进行异丙醇-丁醇-乙醇发酵的溶剂梭菌。通过研究其在不同碳水化合物上的生长情况，我们验证了它利用甘油生产 1,3-丙二醇和异丙醇的能力。全基因组测序表明，其基因组为单一圆形染色体，属于梭菌属 I 簇（严格意义上）。通过培养分析，我们突出了与两种选定的密切相关菌株相比，其特定的行为。尽管发现了几个 CRISPR 基因座，但 16 个推定的原噬菌体显示出接收外源 DNA 的能力。因此，该菌株具有未来工程改造其 1,3-丙二醇生物合成途径和可能在生物燃料生产中工业利用的必要特征。
