Chen Chaoyang, Sun Chongran, Wu Yi-Rui
Department of Biology, Shantou University, Shantou, 515063, Guangdong, China.
STU-UNIVPM Joint Algal Research Center, Shantou University, Shantou, 515063, Guangdong, China.
Curr Microbiol. 2018 Aug;75(8):1011-1015. doi: 10.1007/s00284-018-1481-5. Epub 2018 Mar 21.
A wild-type solventogenic strain Clostridium diolis WST, isolated from mangrove sediments, was characterized to produce high amount of butanol and acetone with negligible level of ethanol and acids from glucose via a unique acetone-butanol (AB) fermentation pathway. Through the genomic sequencing, the assembled draft genome of strain WST is calculated to be 5.85 Mb with a GC content of 29.69% and contains 5263 genes that contribute to the annotation of 5049 protein-coding sequences. Within these annotated genes, the butanol dehydrogenase gene (bdh) was determined to be in a higher amount from strain WST compared to other Clostridial strains, which is positively related to its high-efficient production of butanol. Therefore, we present a draft genome sequence analysis of strain WST in this article that should facilitate to further understand the solventogenic mechanism of this special microorganism.
从红树林沉积物中分离出的野生型产溶剂菌株嗜二醇梭菌WST，其特征在于通过独特的丙酮 - 丁醇（AB）发酵途径从葡萄糖中产生大量丁醇和丙酮，而乙醇和酸的含量可忽略不计。通过基因组测序，菌株WST的组装草图基因组计算为5.85 Mb，GC含量为29.69%，包含5263个基因，这些基因有助于对5049个蛋白质编码序列进行注释。在这些注释基因中，与其他梭菌菌株相比，菌株WST中的丁醇脱氢酶基因（bdh）数量更多，这与其高效生产丁醇呈正相关。因此，我们在本文中展示了菌株WST的草图基因组序列分析，这应有助于进一步了解这种特殊微生物的产溶剂机制。
