Schoch Teresa, Baur Tina, Kunz Johanna, Stöferle Sophia, Dürre Peter
Institut für Mikrobiologie und Biotechnologie, Universität Ulm, 89081 Ulm, Germany.
Microorganisms. 2023 Mar 18;11(3):784. doi: 10.3390/microorganisms11030784.
1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as environmental issues. An alternative is the biobased fermentation of 1,3-PDO from cheap glycerol. DSM 6423 was originally reported to produce 1,3-PDO. However, this could not be confirmed, and a genome analysis revealed the loss of an essential gene. Thus, 1,3-PDO production was genetically reinstalled. Genes for 1,3-PDO production from DSM 525 and DSM 15410 (formerly ) were introduced into DSM 6423 to enable 1,3-PDO production from glycerol. 1,3-PDO production by recombinant strains were investigated under different growth conditions. 1,3-PDO production was only observed for [pMTL83251_P_1,3-PDO.diolis], which harbors the genes of DSM 15410. By buffering the growth medium, production could be increased by 74%. Furthermore, the effect of four different promoters was analyzed. The use of the constitutive promoter from led to a 167% increase in 1,3-PDO production compared to the initial recombinant approach.
1,3 - 丙二醇（1,3 - PDO）是一种有价值的基础化学品，尤其在聚合物工业中用于生产聚对苯二甲酸丙二醇酯。不幸的是，1,3 - PDO的生产主要依赖石油产品作为前体。此外，化学合成路线存在显著缺点，如环境问题。一种替代方法是由廉价甘油进行1,3 - PDO的生物基发酵。最初报道DSM 6423能生产1,3 - PDO。然而，这一点无法得到证实，基因组分析显示一个必需基因缺失。因此，从基因层面重新赋予了DSM 6423生产1,3 - PDO的能力。将来自DSM 525和DSM 15410（原 ）的1,3 - PDO生产基因导入DSM 6423，使其能够从甘油生产1,3 - PDO。对重组菌株在不同生长条件下的1,3 - PDO生产情况进行了研究。仅在含有DSM 15410基因的[pMTL83251_P_1,3 - PDO.diolis]中观察到了1,3 - PDO的生产。通过缓冲生长培养基，产量可提高74%。此外，分析了四种不同启动子的效果。与最初的重组方法相比，使用来自 的组成型启动子使1,3 - PDO产量提高了167%。
