Fujiwara K T, Ashida K, Nishina H, Iba H, Miyajima N, Nishizawa M, Kawai S
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
J Virol. 1987 Dec;61(12):4012-8. doi: 10.1128/JVI.61.12.4012-4018.1987.
Using a DNA probe from an avian transforming virus, NK24, that contains the fos gene and that was newly isolated and characterized (M. Nishizawa, N. Goto, and S. Kawai, J. Virol. 61:3733-3740, 1987), we cloned and sequenced the chicken c-fos gene. Results showed that this gene, like the mouse or human c-fos gene, contains four exons encoding a predicted gene product of 367 amino acids which is about 79% homologous to p55c-fos (mouse), excluding several small insertions or gaps. The predicted gene product, however, contains two nonhomologous regions, as suggested by sequence analysis of the NK24 genome. About half the identical amino acids of these gene products are encoded by different codons, and the guanine-plus-cytosine content in the third letter of each codon used in the entire chicken c-fos coding region is high (93%). The 5'-flanking region of the TATA box contains a sequence that is homologous to a putative transcriptional regulatory sequence in the mouse or human c-fos gene. The 3'-noncoding region of chicken c-fos is strikingly homologous to that of mouse or human c-fos and covers a 67-base-pair adenine-plus-thymine-rich stretch which was previously reported to be essential for an inhibitory effect on the expression of the mouse c-fos gene.
我们使用来自一种禽转化病毒NK24的DNA探针，该探针含有fos基因，是新分离并鉴定的（M. Nishizawa、N. Goto和S. Kawai，《病毒学杂志》61:3733 - 3740，1987），克隆并测序了鸡的c - fos基因。结果表明，该基因与小鼠或人类的c - fos基因一样，包含四个外显子，编码一个预测的由367个氨基酸组成的基因产物，该产物与p55c - fos（小鼠）约79%同源，不包括几个小的插入或缺口。然而，根据NK24基因组的序列分析，预测的基因产物包含两个非同源区域。这些基因产物大约一半的相同氨基酸由不同密码子编码，并且在整个鸡c - fos编码区域中使用的每个密码子的第三个字母中的鸟嘌呤加胞嘧啶含量很高（93%）。TATA框的5'侧翼区域包含一个与小鼠或人类c - fos基因中一个假定的转录调控序列同源的序列。鸡c - fos的3'非编码区域与小鼠或人类的c - fos的序列惊人地同源，并且覆盖了一个67个碱基对的富含腺嘌呤加胸腺嘧啶的片段，该片段先前据报道对小鼠c - fos基因的表达抑制作用至关重要。
