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对FBJ-鼠肉瘤病毒前病毒和c-fos(小鼠)基因的分析表明,病毒和细胞的fos基因产物具有不同的羧基末端。

Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini.

作者信息

Van Beveren C, van Straaten F, Curran T, Müller R, Verma I M

出版信息

Cell. 1983 Apr;32(4):1241-55. doi: 10.1016/0092-8674(83)90306-9.

Abstract

The complete nucleotide sequence of the FBJ-MuSV proviral DNA and the cellular homolog (c-fos) of its oncogene (v-fos) have been determined. The 4026 nucleotide long FBJ-MuSV proviral DNA contains two long terminal repeats, a substitution of 1639 nucleotides of mouse cellular DNA (v-fos) and the 3' end of the env gene derived from FBJ-MuLV. The sequences of the parental FBJ-MuLV and the cellular c-fos (mouse) gene share five of five nucleotides at the 5' end and ten of 11 nucleotides at the 3' end of the v-fos substitution. When compared with the v-fos sequences, the c-fos gene contains four discontinuous regions, three of which are flanked by sequences characteristic of introns. Direct sequence analysis of c-fos (mouse) RNA by primer extension demonstrates that the fourth discontinuity is due to a 104 bp deletion in the v-fos gene. As a consequence of the deletion, the predicted v-fos and c-fos gene products differ at their C termini.

摘要

已确定FBJ-MuSV前病毒DNA的完整核苷酸序列及其癌基因(v-fos)的细胞同源物(c-fos)。长度为4026个核苷酸的FBJ-MuSV前病毒DNA包含两个长末端重复序列、一段1639个核苷酸的小鼠细胞DNA(v-fos)替代序列以及源自FBJ-MuLV的env基因3'端。亲代FBJ-MuLV与细胞c-fos(小鼠)基因的序列在v-fos替代序列的5'端五个核苷酸中有五个相同,在3'端11个核苷酸中有十个相同。与v-fos序列相比,c-fos基因包含四个不连续区域,其中三个区域两侧是内含子特征性序列。通过引物延伸对c-fos(小鼠)RNA进行直接序列分析表明,第四个不连续区域是由于v-fos基因中104 bp的缺失。由于该缺失,预测的v-fos和c-fos基因产物在其C末端有所不同。

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