Roberson Zackery R, Goodpaster John V
Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, 402 North Blackford St., LD326, Indianapolis, IN, 46202, United States.
Department of Chemistry and Chemical Biology, Indiana University Purdue University Indianapolis, 402 North Blackford St., LD326, Indianapolis, IN, 46202, United States; Forensic and Investigative Sciences Program, Indiana University Purdue University Indianapolis, 402 North Blackford St., LD326, Indianapolis, IN, 46202, United States.
Talanta. 2021 Jan 15;222:121461. doi: 10.1016/j.talanta.2020.121461. Epub 2020 Aug 13.
Gas Chromatography-Vacuum UV Spectroscopy (GC-VUV) has seen increased attention in many areas, however, a statistical optimization of VUV method parameters has not been published. This article presents the first statistical optimization of parameters influencing analytes such as cocaine in the VUV flow-cell. Flow-cell temperature, make-up gas pressure, and carrier gas flow rate from the GC were examined and optimized for the detection of controlled substances. The accuracy, precision, linearity, and optimized detection limits for drugs such as cocaine (98.5%, 1.2%, 0.9998, 1.5 ng), heroin (99.3%, 0.94%, 0.9998, 2.0 ng), and fentanyl (98.5%, 1.7%, 0.9752, 9.7 ng) are reported. In general, the limits of detection for cocaine, heroin, fentanyl, and methamphetamine after optimization were comparable to gas chromatography-mass spectrometry (GC-MS) in "scan mode", which had detection limits of 1.1-38 ng on column. The VUV absorption spectra of cocaine, PCP, lorazepam, and HU-210 are also reported. And three samples of "real world" cocaine are analyzed to demonstrate applicability to forensic drug analysis.
气相色谱 - 真空紫外光谱法（GC - VUV）在许多领域受到了越来越多的关注，然而，尚未有关于VUV方法参数的统计优化的报道。本文首次对影响VUV流通池内可卡因等分析物的参数进行了统计优化。对流通池温度、补充气压力和来自气相色谱的载气流速进行了研究，并针对受控物质的检测进行了优化。报告了可卡因（98.5%，1.2%，0.9998，1.5纳克）、海洛因（99.3%，0.94%，0.9998，2.0纳克）和芬太尼（98.5%，1.7%，0.9752，9.7纳克）等药物的准确度、精密度、线性和优化后的检测限。总体而言，优化后可卡因、海洛因、芬太尼和甲基苯丙胺的检测限与气相色谱 - 质谱法（GC - MS）“扫描模式”下的检测限相当，后者在柱上的检测限为1.1 - 38纳克。还报告了可卡因、苯环己哌啶、劳拉西泮和HU - 210的VUV吸收光谱。并且分析了三个“实际”可卡因样品，以证明其在法医毒品分析中的适用性。
