Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, Institute for Advanced Interdisciplinary Research, University of Jinan, Jinan, 250022, PR China; Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, PR China.
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Talanta. 2021 Jan 15;222:121517. doi: 10.1016/j.talanta.2020.121517. Epub 2020 Aug 15.
A direct-readout photoelectrochemical (PEC) lab-on-paper device based on coupled an electricity generating system and paper supercapacitors was established for highly sensitive detection of adenosine triphosphate (ATP). Concretely, CdSe quantum dots (QDs) decorated ZnO networks assembled sensing surface provided outstanding photoelectric properties, on which glucose oxidase (GOx) labeled aptamer was subsequently immobilized via the hybridization chain reaction. With analytes present, specific recognition was stimulated by aptamer, resulting in labeled GOx released. Such released GOx could flow to electrochemical cell to conduct electrochemical redox reactions, which could effectively produce electricity that was stored by capacitor I. Sequentially, photoactive material produced an outstanding voltage due to the decrease of steric hindrance on the sensing interface, which was utilized for charging an external capacitor II. The two instantaneous current was acquired along with the discharge of capacitor I and II by digital multimeter (DMM) readout, respectively. The summational current values performed an increment in pace with the addition of target ATP concentration with the dynamic working range from 10 nM to 3 μM and a detection limit of 6.3 nM attained. Significantly, the signal amplified strategy utilizing as-generated electricity from electrochemical redox reactions were isolated from the photoelectrodes, which was beneficial for amplifying the signal response in the PEC matrices and the development of more efficient signal performance.
建立了基于耦合发电系统和纸超级电容器的直读光电化学（PEC）纸上实验室装置，用于对三磷酸腺苷（ATP）进行高灵敏度检测。具体来说，CdSe 量子点（QD）修饰的 ZnO 网络组装的传感表面提供了出色的光电性能，随后通过杂交链式反应将葡萄糖氧化酶（GOx）标记的适体固定在其上。存在分析物时，通过适体刺激特异性识别，导致标记的 GOx 释放。这种释放的 GOx 可以流向电化学电池以进行电化学氧化还原反应，这可以有效地产生由电容器 I 存储的电流。随后，由于传感界面上的空间位阻减小，光活性材料产生了出色的电压，该电压用于为外部电容器 II 充电。通过数字多用表（DMM）读取，分别获得了随着电容器 I 和 II 的放电而获得的两个瞬时电流。随着目标 ATP 浓度的增加，总和电流值呈递增趋势，动态工作范围从 10 nM 到 3 μM，检测限达到 6.3 nM。重要的是，利用电化学氧化还原反应产生的电能的信号放大策略与光电电极分离，这有利于在 PEC 基质中放大信号响应并开发更有效的信号性能。
