Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic.
Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech Republic.
Molecules. 2020 Nov 5;25(21):5143. doi: 10.3390/molecules25215143.
Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera and were identified by the MALDI-TOF MS and biochemical methods. The produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV-Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.
从 2017 年在乳制品厂各个地点采集的 6 份乳制品工业废水样本中，分离出了 86 株具有脱羧酶活性的细菌菌株。通过 MALDI-TOF MS 和生化方法，从废水样本中鉴定出属 和 种的物种。采用 HPLC/UV-Vis 法检测了八种生物胺（BAs）的产生量。所有分离出的细菌都能够产生四种到八种 BAs。酪胺、腐胺和尸胺属于最常产生的 BAs。在所分离的细菌中，有 41%的细菌能够产生>100mg/L 的 BAs。因此，废水体现了具有脱羧酶阳性微生物传播潜力的载体，这在食品安全控制的危害分析中应予以考虑。还监测了该废水的参数（亚硝酸盐、硝酸盐、磷酸盐和蛋白质含量）。
