Gutiérrez-Cárdenas Oscar Giovanni, Adán Ángeles, Beperet Inés, Medina Pilar, Caballero Primitivo, Garzón Agustín
Crop Protection Unit, School of Agricultural, Food and Biosystems Engineering, Technical University of Madrid, Puerta de Hierro, 2, 28040 Madrid, Spain.
Research & Development Department, Bioinsectis SL. Pol. Ind. Mocholi Plaza Cein 5, Nave A14, 31110 Noain, Navarra, Spain.
Insects. 2020 Nov 5;11(11):760. doi: 10.3390/insects11110760.
Baculoviruses (BV) are highly effective against lepidopteran pests of economic importance such as . The combined use of entomopathogens and macrobiological control agents requires the study of their relationships. Laboratory bioassays were developed to evaluate the interactions between the multiple nucleopolyhedroviruses of (SeMNPV) and (AcMNPV), and the predator . The microscopic examination of predator's excreta (larval drops and meconia) after the ingestion of BV-infected revealed the presence of viral occlusion bodies (OBs). The reinfection of larvae with BVs-contaminated excreta by using OBs water suspensions or by direct application both yielded high mortality values but different speed-of-kill results. Meconia killed before in suspensions due to their higher viral load and larval excretion drops did so in direct application due to their liquid nature and their easiness of consumption. The prey-mediated ingestion of SeMNPV and AcMNPV triggered slight effects in , which were probably derived from the food nutritional quality. larvae did not discriminate between healthy and BV-infected , while a preference was shown for (healthy or infected) vs. . Our findings present , and particularly its larvae, as a promissory candidate for BV dispersion in the field.
杆状病毒（BV）对诸如经济上重要的鳞翅目害虫非常有效。昆虫病原体和宏观生物控制剂的联合使用需要研究它们之间的关系。已开展实验室生物测定以评估苜蓿银纹夜蛾多核多角体病毒（SeMNPV）和苜蓿丫纹夜蛾多核多角体病毒（AcMNPV）这两种多核多角体病毒与捕食者之间的相互作用。在捕食者摄入感染BV的猎物后，对其排泄物（幼虫粪便和蛹便）进行显微镜检查，发现了病毒包涵体（OBs）。通过使用OBs水悬浮液或直接应用，用受BV污染的排泄物对猎物幼虫进行再感染均产生了高死亡率，但致死速度结果不同。蛹便由于其较高的病毒载量在悬浮液中先于幼虫粪便致死，而幼虫粪便由于其液体性质和易于被消耗在直接应用中先于蛹便致死。猎物介导的SeMNPV和AcMNPV摄入对捕食者产生了轻微影响，这可能源于食物的营养质量。猎物幼虫在健康猎物和感染BV的猎物之间没有表现出区分，而对（健康或感染的）猎物与其他猎物相比表现出偏好。我们的研究结果表明，捕食者，特别是其幼虫，是田间BV传播的有前景的候选者。
