Zeitz Johanna O, Ehbrecht Tamara, Fleischmann Anne, Most Erika, Gessner Denise K, Friedrichs Silvia, Sparenberg Marion, Failing Klaus, Whelan Rose, Lütjohann Dieter, Eder Klaus
Institute of Animal Nutrition and Nutritional Physiology, Justus Liebig University, D-35392 Giessen, Germany.
Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, D-53127 Bonn, Germany.
Animals (Basel). 2020 Nov 5;10(11):2050. doi: 10.3390/ani10112050.
In this study, the hypothesis that supplementation with methionine (Met) as DL-Met (DLM) in excess of the National Research Council (NRC) recommendations improves the antioxidant system in broilers was investigated. Day-old male Cobb-500 broilers (n = 72) were divided into three groups which were fed a control diet or diets supplemented with two levels of DLM in which the concentrations of Met + Cys exceeded the recommendations of NRC by 15-20% (group DLM 1) or 30-40% (group DLM 2), respectively. The three groups of broilers did not show differences in body weight gains, feed intake, and feed conversion ratio. However, broilers of groups DLM 1 and DLM 2 had higher concentrations of glutathione (GSH) in liver and thigh muscle and lower concentrations of cholesterol oxidation products (COPs) in heat-processed thigh muscle than broilers of the control group. Concentrations of several oxidation products of phytosterols in heat-processed thigh muscle were also reduced in groups DLM 1 and DLM 2; however, the concentration of total oxidation products of phytosterols was not different between the three groups. The study shows that DLM supplementation improved the antioxidant status due to an increased formation of GSH and reduced the formation of COPs during heat-processing in thigh muscle.
在本研究中，探讨了以超过美国国家研究委员会（NRC）建议量的DL-蛋氨酸（DLM）形式补充蛋氨酸（Met）可改善肉鸡抗氧化系统的假说。将1日龄雄性科宝500肉鸡（n = 72）分为三组，分别饲喂对照日粮或补充两种水平DLM的日粮，其中蛋氨酸+半胱氨酸的浓度分别超过NRC建议量的15-20%（DLM 1组）或30-40%（DLM 2组）。三组肉鸡在体重增加、采食量和饲料转化率方面未表现出差异。然而，与对照组肉鸡相比，DLM 1组和DLM'组肉鸡肝脏和大腿肌肉中的谷胱甘肽（GSH）浓度更高，热加工大腿肌肉中的胆固醇氧化产物（COPs）浓度更低。DLM 1组和DLM 2组热加工大腿肌肉中几种植物甾醇氧化产物的浓度也有所降低；然而，三组之间植物甾醇总氧化产物的浓度没有差异。该研究表明，补充DLM可通过增加GSH的形成来改善抗氧化状态，并减少大腿肌肉热加工过程中COPs的形成。
