School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia; Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2650, Australia.
School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia.
Food Chem. 2017 Sep 1;230:195-207. doi: 10.1016/j.foodchem.2017.02.127. Epub 2017 Feb 28.
The thiobarbituric acid reactive substances (TBARS) assay is widely used to measure lipid oxidation and antioxidant activity in food and physiological systems. However, there has been no review (to our knowledge) that focuses exclusively on this test. This review presents an overview of the current use of the TBARS test in food and physiological systems, before looking at the various ways in which the assay is used in studies on antioxidant activity. As an antioxidant assay, the TBARS test may lack acceptable reproducibility, and long reaction times may preclude its adoption as a rapid screening method. Despite these potential limitations, there are features of the TBARS test that make it useful as a complement to popular screening tests such as Trolox equivalent antioxidant capacity. This review concludes with proposals for development of the TBARS test so that it can be used as a rapid and robust antioxidant assay.
硫代巴比妥酸反应物质（TBARS）测定法广泛用于测量食品和生理系统中的脂质氧化和抗氧化活性。然而，据我们所知，目前还没有专门针对该测试的综述。本综述介绍了 TBARS 测试在食品和生理系统中的当前用途，然后研究了该测定法在抗氧化活性研究中的各种应用。作为一种抗氧化剂测定法，TBARS 测试可能缺乏可接受的重现性，并且较长的反应时间可能会阻止其作为快速筛选方法采用。尽管存在这些潜在的局限性，但 TBARS 测试的某些特性使其可作为 Trolox 等效抗氧化能力等流行筛选测试的补充方法。本综述最后提出了对 TBARS 测试的发展建议，以便将其用作快速而稳健的抗氧化剂测定法。
