Graduate School of Health Sciences, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
Secretion Biology Laboratory, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan; Center for Food Science and Wellness, Gunma University, 4-2 Aramaki, Maebashi, Gunma 371-8510, Japan.
Food Chem. 2021 May 1;343:128511. doi: 10.1016/j.foodchem.2020.128511. Epub 2020 Nov 1.
Inhibitors against cystine-glutamate antiporter, including erastin, elicit ferroptotic cell death. The erastin-induced ferroptotic cell death appears to be caused by cysteine as well as glutathione depletion. Cysteine is an essential substrate for sulfane sulfur producing systems in cells, generating persulfides that function as intracellular antioxidants and intermediates in iron-sulfur cluster production. Therefore, we examined whether botanical sulfane sulfur donors such as diallyl trisulfide (DATS) and dimethyl trisulfide (DMTS) prevent ferroptotic cell death in HT1080 cells treated with erastin. As a result, DMTS (20 μM) and DATS (10 μM) rescued the erastin-treated HT1080 cells by 69.6% and 91.6%, respectively. Furthermore, DMTS-containing squeeze of cabbage (2.0 g/L) and DATS-containing squeeze of garlic (0.07 g/L) rescued the erastin-treated HT1080 cells by 76.5% and almost 100%, respectively. In conclusion, the ingestion of trisulfides may bring about increased resistance to ferroptotic cell death in vivo.
胱氨酸-谷氨酸反向转运体抑制剂，包括 erastin，可引发铁死亡细胞死亡。erastin 诱导的铁死亡细胞死亡似乎是由半胱氨酸和谷胱甘肽耗竭引起的。半胱氨酸是细胞中产生硫磺酸的含硫系统的必需底物，生成的过硫化物作为细胞内抗氧化剂和铁硫簇生成的中间产物。因此，我们研究了植物硫磺酸供体，如二烯丙基三硫化物（DATS）和二甲基三硫化物（DMTS），是否能预防 erastin 处理的 HT1080 细胞中的铁死亡。结果表明，DMTS（20 μM）和 DATS（10 μM）分别挽救了 69.6%和 91.6%的 erastin 处理的 HT1080 细胞。此外，白菜中的 DMTS 挤压物（2.0 g/L）和大蒜中的 DATS 挤压物（0.07 g/L）分别挽救了 76.5%和几乎 100%的 erastin 处理的 HT1080 细胞。总之，摄入三硫化物可能会增加体内对铁死亡细胞死亡的抵抗力。
