Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil; Professor in University Centre Integrated Faculties of Ourinhos, BR-153, Km 338 S/N - Água Do Cateto, Ourinhos, SP, 19909-100, Brazil.
Department of Biomaterials and Biomechanics, Oregon Health and Science University, OHSU, 2730 SW Moody Ave, Portland, OR, 97201, USA.
J Mech Behav Biomed Mater. 2021 Feb;114:104177. doi: 10.1016/j.jmbbm.2020.104177. Epub 2020 Oct 27.
The aim of this study was to explore the impact of the interaction between an MDP-based universal adhesive system in etch-and-rinse mode and two proteolytic inhibitors on the longevity of restorations bonded to artificially-affected-dentin substrates. 90 sound human third molars were randomly distributed into three groups according to the substrate: N-no challenges-control (stored in artificial saliva), ACD-artificial caries dentin (6 h DE + 18 h-RE/5 days + 48 h RE) and ERO-artificial erosion dentin (3 × 5 min/5 days with orange juice). They were further redistributed according to dentin pretreatment: W- water (control), CHX-2% digluconate chlorhexidine and E64- 5 μM E64-Trans-Epoxysuccinyl-L-Leucylamido-(4-guanidino) butane, which resulted in the following 9 groups (n = 10): N-W, N-CHX, N-E64, ACD-W, ACD-CHX, ACD-E64, ERO-W, ERO-CHX and ERO-E64. All specimens were restored with Adper Single Bond Universal (Etch-and-rinse mode)/Filtek Z250. Sticks (0.64 mm) were obtained and subjected to microtensile test (μTBS) in a universal testing machine at 0.5 mm/min for 7-days, 6 and 18-month analyses. Failure modes were classified using optical microscopy (40X). Data were statistically analyzed by three-way ANOVA and Tukey tests (p < 0.05). All individual factors (p < 0.0001) and interaction between factors were statistically significant (substrate X pretreatment (p = 0.00093); substrate X time (p = 0.01035) and pretreatment X time (p = 0.0035). Caries-affected substrate was the most compromised one, disregarding the pretreatment. CHX was mostly affected compared with E64 up to 18 months, possibly due to its calcium-dependent mechanism.
本研究旨在探讨基于 MDP 的通用酸蚀黏结剂与两种蛋白水解抑制剂在人工酸蚀牙本质底物上的交互作用对修复体长期留存效果的影响。90 颗健康的第三磨牙随机分为三组，根据底物分为：N-无挑战对照（储存在人工唾液中）、ACD-人工龋牙本质（6 h DE+18 h-RE/5 天+48 h RE）和 ERO-人工酸蚀牙本质（用橙汁进行 3×5 分钟/5 天）。根据牙本质预处理进一步分为：W-水（对照）、CHX-2%葡萄糖酸氯己定和 E64-5 μM E64-反式-环氧琥珀酰基-L-亮氨酰氨基-(4-胍基)丁烷，共分为 9 组（n=10）：N-W、N-CHX、N-E64、ACD-W、ACD-CHX、ACD-E64、ERO-W、ERO-CHX 和 ERO-E64。所有样本均用 Adper Single Bond Universal（酸蚀冲洗模式）/Filtek Z250 进行修复。制备 0.64mm 棒状试件，在万能试验机上以 0.5mm/min 的速度进行微拉伸试验（μTBS），分别在 7 天、6 个月和 18 个月进行分析。使用光学显微镜（40X）对失效模式进行分类。采用三因素方差分析和 Tukey 检验对数据进行统计学分析（p<0.05）。所有个体因素（p<0.0001）和因素间的交互作用均具有统计学意义（底物 X 预处理（p=0.00093）；底物 X 时间（p=0.01035）和预处理 X 时间（p=0.0035）。与预处理相比，龋损底物对牙体的影响最大。与 E64 相比，CHX 在 18 个月时受到的影响最大，这可能是由于其钙依赖性机制。
