Restorative Dentistry Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt.
Oper Dent. 2013 Jul-Aug;38(4):439-46. doi: 10.2341/12-251-L. Epub 2013 Jan 17.
To evaluate the bond strength durability of a single-step self-etch adhesive to dentin pretreated with either ethanol-based chlorhexidine (ECHX) or water-based chlorhexidine (WCHX) after storage in artificial saliva and under intrapulpal pressure simulation (IPPS).
The occlusal enamel of 30 freshly extracted premolars was trimmed to expose midcoronal dentin. Roots were sectioned to expose the pulp chamber. Specimens were distributed over three groups (n=10) according to the dentin pretreatment used. In the first group, Adper Easy One (3M ESPE) was applied to the dentin surfaces according to the manufacturer's instructions (control group). In the second group, dentin was pretreated before bonding with 1 mL of 2% CHX diacetate dissolved in 100% ethanol (ECHX). The third group received the same pretreatment; however, CHX was dissolved in distilled water (WCHX). Pretreatment and bonding were carried out while the specimens were subjected to IPPS. Resin composite (Valux Plus, 3MESPE) buildups were made. After curing, specimens were stored in artificial saliva and under IPPS at 37°C in a specially constructed incubator (n=5/group) either for 24 hours or six months prior to testing. Thereafter, bonded specimens were sectioned into sticks with a cross section of 0.9 ± 0.01 mm(2) and subjected to microtensile bond strength (μTBS) testing (n=25/subgroup) using a universal testing machine. Data were statistically analyzed using two-way analysis of variance (ANOVA) with repeated measures, one-way ANOVA , and Bonferroni post hoc tests (p≤0.05). Failure modes were determined using a scanning electron microscope.
After 24 hours of storage, control and WCHX groups revealed significantly higher μTBS than the ECHX group. After six-month storage in artificial saliva and IPPS, only the WCHX group maintained its μTBS value. The predominant mode of failure was the mixed type, except for the ECHX group, which was mostly adhesive.
Pretreatment of the dentin with ECHX had a negative effect on bonding of the tested single-step self-etch adhesive; however, WCHX showed bond stability under IPPS.
评估经乙醇基氯己定（ECHX）或水基氯己定（WCHX）预处理牙本质后，单步自酸蚀黏结剂在人工唾液储存和牙髓内压模拟（IPPS）下的黏结强度耐久性。
30 颗新鲜拔除的前磨牙的咬合釉质被修剪以暴露近中牙本质。根部分为两部分以暴露牙髓腔。根据牙本质预处理方法将标本分为三组（每组 n=10）。在第一组中，根据制造商的说明将 Adper Easy One（3M ESPE）应用于牙本质表面（对照组）。在第二组中，在黏合前用 1 mL 2% CHX 二乙酸溶解于 100%乙醇（ECHX）预处理牙本质。第三组接受相同的预处理；然而，CHX 溶解在蒸馏水中（WCHX）。预处理和黏合在对标本进行 IPPS 的同时进行。用树脂复合材料（Valux Plus，3M ESPE）进行堆积。固化后，将标本在人工唾液中储存，并在 37°C 的特殊构造孵育器中进行 IPPS（每组 n=5），分别储存 24 小时或 6 个月后进行测试。然后，将黏合的标本切成横截面为 0.9±0.01mm(2)的棒状，并使用万能试验机进行微拉伸黏结强度（μTBS）测试（每组 n=25）。使用双向方差分析（ANOVA）、单向方差分析和 Bonferroni 事后检验（p≤0.05）对数据进行统计分析。使用扫描电子显微镜确定失效模式。
储存 24 小时后，对照组和 WCHX 组的 μTBS 明显高于 ECHX 组。在人工唾液和 IPPS 中储存 6 个月后，只有 WCHX 组保持其 μTBS 值。主要失效模式为混合模式，除 ECHX 组外，其余均为黏附性失效。
ECHX 预处理牙本质对测试的单步自酸蚀黏结剂的黏结有负面影响；然而，WCHX 在 IPPS 下显示出黏结稳定性。
