Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Department of Surgery, Stomatology, Pathology, and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
Clin Oral Investig. 2024 Oct 25;28(11):611. doi: 10.1007/s00784-024-05994-8.
Self-etching dental adhesives bond with dentin through chemical reactions with calcium. This study assessed bond strength (BS) using microtensile (µTBS) and microshear (µSBS) tests on sound and post-radiotherapy dentin, with dental adhesives containing different functional monomers.
Sound dentin (SD) and post-radiotherapy irradiated dentin (ID) were tested with two adhesive systems: Clearfil SE Bond (SE, 10-MDP-based) and FL Bond II (FL, containing carboxylic and phosphonic monomers with S-PRG bioactive particles). The tests occurred initially (24 h) and six months later; fracture mode was also analyzed (40x). Ninety-six human molars were randomly assigned (n = 12), and half were irradiated with a 70 Gy radiation dose. For µTBS test, teeth were bonded, restored and sectioned them into beams (0.64 mm). The µSBS test used filled transparent cylindrical matrices with resin composite and light-cured them after dental adhesive applications. Three-way ANOVA and Tukey's test (p < 0.05) analyzed the data.
µTBS showed a significant substrate x adhesive interaction (p < 0.001), while µSBS was significant for all factors (p = 0.006). SE and FL performed better on SD and ID, respectively, in the µTBS test. As for µSBS, SE showed higher values on ID (p < 0.05). Lower BS values occurred for SD-FL and ID-SE after six months.
Dental adhesive performance varied based on substrate type and test method. FL was more stable for ID in µTBS, while SE excelled in µSBS.
As post-radiotherapy irradiated dentin becomes more vulnerable, self-etching systems based on functional monomer and bioactive ingredients may exhibit appropriate bonding to this altered substrate.
自酸蚀牙本质粘结剂通过与钙离子的化学反应与牙本质结合。本研究使用微拉伸（µTBS）和微剪切（µSBS）试验评估了不同功能单体的含自酸蚀牙本质粘结剂在正常牙本质和放疗后牙本质上的粘结强度（BS）。
使用两种粘结系统对正常牙本质（SD）和放疗后牙本质（ID）进行测试：Clearfil SE Bond（SE，基于 10-MDP）和 FL Bond II（FL，含有羧酸和膦酸单体以及 S-PRG 生物活性颗粒）。试验初始（24 h）和 6 个月后进行；还分析了断裂模式（40x）。96 个人类磨牙随机分配（n=12），其中一半用 70 Gy 剂量照射。对于 µTBS 试验，将牙齿粘结、修复并将其切成梁（0.64 mm）。µSBS 试验使用填充透明圆柱形基质和树脂复合材料，在牙本质粘结剂应用后用光固化。三因素方差分析和 Tukey 检验（p<0.05）分析数据。
µTBS 显示基底 x 粘结剂的交互作用有显著差异（p<0.001），而 µSBS 对所有因素均有显著差异（p=0.006）。SE 和 FL 在 µTBS 试验中分别在 SD 和 ID 上表现更好。对于 µSBS，SE 在 ID 上的数值更高（p<0.05）。6 个月后，SD-FL 和 ID-SE 的 BS 值较低。
牙本质粘结剂的性能取决于基底类型和试验方法。FL 在 µTBS 中对 ID 更稳定，而 SE 在 µSBS 中表现更好。
随着放疗后牙本质变得更加脆弱，基于功能单体和生物活性成分的自酸蚀系统可能对这种改变的基底表现出适当的粘结。
