Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China.
Scand J Gastroenterol. 2020 Dec;55(12):1467-1474. doi: 10.1080/00365521.2020.1839961. Epub 2020 Nov 10.
Esophageal bacteria, as the integral composition of human ecosystem, have been reported to be associated with esophageal lesions. However, few studies focus on microbial compositions in different esophageal segments, especially after Lugol's iodine staining (LIS) in the endoscopic examination for the screening of esophageal cancer. We aim to investigate the composition of the bacterial microbiome in upper, middle and lower esophagus and if LIS would affect the detection of bacteria.
A total of 141 fasting samples including the upper, middle and lower esophagus from 27 participants were collected by brushing the mucosal surface of the esophagus before (Eso) and after (Lug) LIS. Bacterial V3-V4 region of 16S rRNA gene was amplified and sequenced by Illumina's sequencing platform.
The top six abundant bacterial phyla taxa among three locations from both Eso and Lug groups were Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria and TM7. In terms of genera, the bacterium in three locations from two groups was all characterized by a highest relative abundance of . Bacteria diversity and the relative abundance between Eso and Lug were comparable ( > .05). Bacteria diversity was consistent in different esophageal locations within the individual.
The bacterial microbiome in healthy esophagus are highly diverse and consistent even among three physiological sites at all clades. Lugol's iodine staining would not change local microenvironment in term of microbial composition. These findings provide an essential baseline for future studies investigating local and systemic bacterial microbiome and esophageal diseases.
食管细菌作为人体生态系统的组成部分之一，已被报道与食管病变有关。然而，很少有研究关注不同食管段的微生物组成，特别是在用于食管癌筛查的内镜检查中经卢戈氏碘染色（LIS）后。我们旨在研究上、中、下食管的细菌微生物组组成，以及 LIS 是否会影响细菌的检测。
共采集 27 名参与者的 141 份空腹样本，包括上、中、下食管，分别在 LIS 前（Eso）和 LIS 后（Lug）用刷子刷取食管黏膜表面。采用 Illumina 测序平台对细菌 16S rRNA 基因的 V3-V4 区进行扩增和测序。
Eso 和 Lug 组三个部位中最丰富的前 6 个细菌门是变形菌门、厚壁菌门、拟杆菌门、放线菌门、梭杆菌门和 TM7。在属水平上，两组三个部位的细菌都以相对丰度最高的特征。细菌多样性和 Eso 与 Lug 之间的相对丰度相当（>0.05）。个体内不同食管部位的细菌多样性一致。
健康食管的细菌微生物组高度多样且一致，即使在三个生理部位的所有分支上也是如此。卢戈氏碘染色不会改变微生物组成方面的局部微环境。这些发现为未来研究局部和全身细菌微生物组与食管疾病提供了重要的基础。
