Department of Chemistry, Fudan University, Shanghai 200433, China.
Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Lab Chip. 2020 Dec 15;20(24):4632-4637. doi: 10.1039/d0lc00677g.
Tumor-derived exosomes, which contain RNA, DNA, and proteins, are a potentially rich non-invasive source of biomarkers. However, no efficient isolation or detection methods are yet available. Here, we developed a microfluidic Raman biochip designed to isolate and analyze exosomes in situ. Anti-CD63 magnetic nanoparticles were used to enrich exosomes through mixing channels of a staggered triangular pillar array. EpCAM-functionalized Raman-active polymeric nanomaterials (Raman beads) allow rapid analysis of exosome samples within 1 h, with a quantitative signal at 2230 cm-1. The limit of detection of this biochip approaches 1.6 × 102 particles per mL with 20 μL samples. The newly developed biochip assay was successfully applied in the determination of exosomes from clinical serum samples. Thus, this novel device may have potential as a clinical exosome analysis tool for prostate cancer.
肿瘤来源的外泌体包含 RNA、DNA 和蛋白质，是一种很有前途的非侵入性生物标志物来源。然而，目前还没有有效的分离或检测方法。在这里，我们开发了一种微流控拉曼生物芯片，旨在原位分离和分析外泌体。通过混合交错三角形柱阵列的通道，使用抗 CD63 磁纳米颗粒来富集外泌体。EpCAM 功能化的拉曼活性聚合物纳米材料（拉曼珠）允许在 1 小时内快速分析外泌体样品，并在 2230 cm-1 处产生定量信号。该生物芯片的检测限接近 1.6×102 个/mL，样品体积为 20 μL。新开发的生物芯片分析成功应用于临床血清样本中外泌体的测定。因此，这种新型装置可能有潜力成为前列腺癌的临床外泌体分析工具。
