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长链非编码RNA LINC01094通过MicroRNA-184上调SLC2A3促进肾透明细胞癌的发展。

Long Non-coding RNA LINC01094 Promotes the Development of Clear Cell Renal Cell Carcinoma by Upregulating SLC2A3 via MicroRNA-184.

作者信息

Xu Haifei, Wang Xiaolin, Wu Jiacheng, Ji Hao, Chen Zhigang, Guo Haifeng, Hou Jianquan

机构信息

Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Department of Urology, Nantong Tumor Hospital, Nantong, China.

出版信息

Front Genet. 2020 Sep 23;11:562967. doi: 10.3389/fgene.2020.562967. eCollection 2020.

DOI:10.3389/fgene.2020.562967
PMID:33173535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7538661/
Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC. Compelling evidence has highlighted the crucial role of long non-coding RNA (lncRNA) in ccRCC. Our current study aims to explore the regulatory mechanism of LINC01094 in the development of ccRCC. Dual-luciferase reporter experiment verified the targeting relationship among miR-184, LINC01094, and SLC2A3. Furthermore, the interaction between LINC01094 and miR-184 was confirmed by RNA immunoprecipitation (RIP) and RNA pull-down. Biological behaviors of ccRCC cells were investigated through cell counting kit-8 (CCK8), scratch test, Transwell, and flow cytometry. The effect of SLC2A3 on the tumorigenicity of nude mice was evaluated . In ccRCC cells and clinical tissues, LINC01094 and SLC2A3 were highly expressed while miR-184 was lowly expressed. Besides, miR-184 was verified to be a direct target of LINC01094. Silencing LINC01094, up-regulating miR-184, or reducing SLC2A3 inhibited the growth, migration, and invasion of ccRCC cells. Tumor growth was suppressed by silenced LINC01215 via reducing the expression of SLC2A3 via miR-184. Taken together, silencing LINC01094 inhibited SLC2A3 expression by up-regulating miR-184, thereby inhibiting the development of ccRCC.

摘要

透明细胞肾细胞癌（ccRCC）是肾细胞癌最常见的亚型。有力证据凸显了长链非编码RNA（lncRNA）在ccRCC中的关键作用。我们当前的研究旨在探索LINC01094在ccRCC发生发展中的调控机制。双荧光素酶报告基因实验验证了miR-184、LINC01094和SLC2A3之间的靶向关系。此外，通过RNA免疫沉淀（RIP）和RNA下拉实验证实了LINC01094与miR-184之间的相互作用。通过细胞计数试剂盒-8（CCK8）、划痕实验、Transwell实验和流式细胞术研究了ccRCC细胞的生物学行为。评估了SLC2A3对裸鼠致瘤性的影响。在ccRCC细胞和临床组织中，LINC01094和SLC2A3高表达，而miR-184低表达。此外，miR-184被证实是LINC01094的直接靶点。沉默LINC01094、上调miR-184或降低SLC2A3可抑制ccRCC细胞的生长、迁移和侵袭。沉默LINC01215通过miR-184降低SLC2A3的表达从而抑制肿瘤生长。综上所述，沉默LINC01094通过上调miR-184抑制SLC2A3表达，从而抑制ccRCC的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/15cb41a84e30/fgene-11-562967-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/21a082168e0d/fgene-11-562967-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/83d72a48a0d6/fgene-11-562967-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/55d655d58e95/fgene-11-562967-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/cf75b616a032/fgene-11-562967-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/085b5a2fadde/fgene-11-562967-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/c523d57e6fb0/fgene-11-562967-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/15cb41a84e30/fgene-11-562967-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/21a082168e0d/fgene-11-562967-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/83d72a48a0d6/fgene-11-562967-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/55d655d58e95/fgene-11-562967-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/cf75b616a032/fgene-11-562967-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/085b5a2fadde/fgene-11-562967-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/c523d57e6fb0/fgene-11-562967-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d3/7538661/15cb41a84e30/fgene-11-562967-g007.jpg

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