LINC01094/miR-577 轴调控卵巢癌的进展。

LINC01094/miR-577 axis regulates the progression of ovarian cancer.

机构信息

Department of Obstetrics and Gynecology, Zibo Hospital of Integrated Traditional Chinese and Western Medicine, Zibo, Shandong Province, China.

Department of Reproductive Medicine, Linyi People's Hospital, Fenghuang Street No. 233, Hedong District, Linyi, Shandong Province, China.

出版信息

J Ovarian Res. 2020 Oct 17;13(1):122. doi: 10.1186/s13048-020-00721-9.

BACKGROUND

Long intergenic non-coding RNA 01094 (LINC01094) is probably a novel regulator in cancer biology. This study aimed to probe into the function and mechanism of LINC01094 in ovarian cancer (OC).

METHODS

Quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to measure LINC01094 and miR-577 expressions in OC tissues and cell lines. Western blot was used to examine the expressions of epithelial-mesenchymal transition (EMT)-related proteins, β-catenin, c-Myc and cyclin D1. Cell counting kit-8 (CCK-8) and Transwell assays were used to detect the proliferation, migration and invasion of SKOV3 and 3AO cells, respectively. Eventually, dual-luciferase reporter gene assay was employed to detect the regulatory relationship between miR-577 and LINC01094.

RESULTS

LINC01094 expression was elevated in OC tissues and cell lines. High LINC01094 expression was associated with higher FIGO stage, lymph node metastasis and the shorter overall survival rate in patients with OC. Meanwhile, LINC01094 knockdown inhibited OC cell proliferation, migration, invasion and EMT. In addition, miR-577 was demonstrated to be a direct downstream target of LINC01094 in OC and inhibition of miR-577 reversed the biological effects of LINC01094 knockdown on OC cells. Additionally, LINC01094 / miR-577 axis regulated the expressions of β-catenin, c-Myc and cyclin D1 in OC cells.

CONCLUSION

LINC01094 promotes the proliferation, migration, invasion and EMT of OC cells by adsorbing miR-577.

背景

长链非编码 RNA 01094（LINC01094）可能是癌症生物学中的一种新型调节因子。本研究旨在探讨 LINC01094 在卵巢癌（OC）中的功能和机制。

方法

利用实时定量聚合酶链反应（qRT-PCR）检测 OC 组织和细胞系中 LINC01094 和 miR-577 的表达。Western blot 检测上皮-间充质转化（EMT）相关蛋白、β-连环蛋白、c-Myc 和细胞周期蛋白 D1 的表达。细胞计数试剂盒-8（CCK-8）和 Transwell 测定分别用于检测 SKOV3 和 3AO 细胞的增殖、迁移和侵袭。最终，双荧光素酶报告基因检测用于检测 miR-577 和 LINC01094 之间的调控关系。

结果

LINC01094 在 OC 组织和细胞系中表达上调。高 LINC01094 表达与 OC 患者 FIGO 分期较高、淋巴结转移和总生存率较短有关。同时，LINC01094 敲低抑制 OC 细胞增殖、迁移、侵袭和 EMT。此外，miR-577 被证明是 OC 中 LINC01094 的直接下游靶标，抑制 miR-577 逆转了 LINC01094 敲低对 OC 细胞的生物学效应。此外，LINC01094/miR-577 轴调节 OC 细胞中β-连环蛋白、c-Myc 和细胞周期蛋白 D1 的表达。