Department of Clinical Laboratory, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
Department of Clinical Laboratory, Shanghai Fourth People's Hospital affiliated to Tongji University School of Medicine, Shanghai 200081, China.
Epigenomics. 2020 Oct;12(19):1707-1723. doi: 10.2217/epi-2019-0365. Epub 2020 Nov 11.
To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection of CCl4. Dynamic m6A methylation was evaluated using MeRIP-Seq in the progression and reversal of hepatic fibrosis at different stages. During the hepatic fibrosis, differential m6A methylation was mainly enriched in processes associated with oxidative stress and cytochrome metabolism, while differential m6A methylation was mainly enriched in processes associated with immune response and apoptosis in the hepatic fibrosis reversal. m6A methylation plays an important role in the progression and reversal of hepatic fibrosis.
为了动态分析肝纤维化进展和逆转过程中的差异 m6A 甲基化。我们通过腹腔注射 CCl4 诱导 C57/BL6 小鼠肝纤维化。连续注射 CCl4 后停药，建立肝纤维化逆转模型。在肝纤维化的不同阶段，使用 MeRIP-Seq 评估进展和逆转过程中的动态 m6A 甲基化。在肝纤维化过程中，差异 m6A 甲基化主要富集在与氧化应激和细胞色素代谢相关的过程中，而在肝纤维化逆转过程中，差异 m6A 甲基化主要富集在与免疫反应和细胞凋亡相关的过程中。m6A 甲基化在肝纤维化的进展和逆转中起着重要作用。
