Shaanxi Provincial Key Laboratory of Biotechnology, Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an, Shaanxi 710069, P. R. China.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, P. R. China.
Chem Commun (Camb). 2020 Nov 26;56(94):14869-14872. doi: 10.1039/d0cc06208a.
Exosomes (a type of nanoscale extracellular vesicle with a size range of 30-100 nm) mediate cell-cell communication by transferring functional biomolecules, and play an important role in various physiological and pathological processes, including tumor development and progression. More new and effective techniques for visualizing and tracking exosomes in cell-cell communication are highly desirable. However, the application of commonly used exosome-labeling probes is limited by the need for specificity and strict pH tolerance. We describe here the construction and testing of a novel exosome labeling fluorescent probe termed as "ExoTracker", which displayed low cytotoxicity and a high fluorescence intensity in acidic environments. ExoTracker was applied for effective tracking of exosomes in cell endocytosis.
外泌体（一种纳米级细胞外囊泡，大小为 30-100nm）通过传递功能生物分子介导细胞间通讯，在包括肿瘤发生和进展在内的各种生理和病理过程中发挥重要作用。因此，非常需要新的和有效的技术来可视化和跟踪细胞间通讯中的外泌体。然而，常用的外泌体标记探针的应用受到特异性和严格 pH 耐受性的限制。我们在这里描述了一种新型外泌体标记荧光探针的构建和测试，称为“ExoTracker”，该探针在酸性环境中表现出低细胞毒性和高荧光强度。ExoTracker 可用于有效跟踪细胞内吞作用中外泌体。
