Han Chaoshan, Qin Gangjian
Department of Biomedical Engineering, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, Birmingham, AL, United States.
Front Cardiovasc Med. 2022 Jun 1;9:922420. doi: 10.3389/fcvm.2022.922420. eCollection 2022.
Extracellular vesicles (EVs) are lipid bilayer particles naturally released from most if not all cell types to mediate inter-cellular exchange of bioactive molecules. Mounting evidence suggest their important role in diverse pathophysiological processes in the development, growth, homeostasis, and disease. Thus, sensitive and reliable assessments of functional EV cargo transfer from donor to acceptor cells are extremely important. Here, we summarize the methods EV are labeled and their functional transfer in acceptor cells are evaluated by various reporter systems.
细胞外囊泡（EVs）是由大多数（即便不是所有）细胞类型自然释放的脂质双层颗粒，用于介导生物活性分子的细胞间交换。越来越多的证据表明它们在发育、生长、体内平衡和疾病等多种病理生理过程中发挥着重要作用。因此，灵敏且可靠地评估功能性细胞外囊泡货物从供体细胞到受体细胞的转移极其重要。在这里，我们总结了细胞外囊泡的标记方法以及通过各种报告系统评估其在受体细胞中的功能转移情况。
