Castagné V, Corder R, Gaillard R, Mormède P
Laboratoire de Psychobiologie des Comportements Adaptatifs, INRA-INSERM U.259, Bordeaux, France.
Regul Pept. 1987 Oct;19(1-2):55-63. doi: 10.1016/0167-0115(87)90074-7.
Circulating concentrations of neuropeptide Y-like immunoreactivity (NPY), noradrenaline (NA) and adrenaline (AD) were measured in conscious, chronically catheterized rats submitted to various stress protocols. Basal plasma levels of NPY, NA and AD (194 +/- 52 fmol/ml, 0.90 +/- 0.11 pmol/ml and 0.52 +/- 0.07 pmol/ml) were increased by handling (+132%, +76% and +629%, respectively) and rose further during electric shock treatment. Adrenalectomy resulted in the complete disappearance of circulating adrenaline but did not alter either control or stress values of noradrenaline. In comparison circulating levels of NPY were reduced, but not significantly in adrenalectomized animals. Insulin stress induced a large increase in plasma AD levels and cold stress induced an increase in plasma NA levels, without any parallel change in NPY concentrations. These results demonstrate that NPY, which is colocalized with catecholamines in the peripheral nervous systems, is also released during stress responses and that its release parallels more closely changes in circulating NA than AD. Furthermore, stress-induced changes in circulating NPY-like immunoreactivity do not originate from the adrenal gland but mainly from the peripheral nervous system, and the release of NPY is dependent upon the nature of the stimulus.
在经长期导管插入术处理的清醒大鼠中，采用各种应激方案，测量了神经肽Y样免疫反应性物质（NPY）、去甲肾上腺素（NA）和肾上腺素（AD）的循环浓度。NPY、NA和AD的基础血浆水平（分别为194±52 fmol/ml、0.90±0.11 pmol/ml和0.52±0.07 pmol/ml）在处理后升高（分别升高132%、76%和629%），并在电击治疗期间进一步升高。肾上腺切除术导致循环肾上腺素完全消失，但未改变去甲肾上腺素的对照值或应激值。相比之下，肾上腺切除动物的NPY循环水平降低，但无显著差异。胰岛素应激导致血浆AD水平大幅升高，冷应激导致血浆NA水平升高，而NPY浓度无相应变化。这些结果表明，在周围神经系统中与儿茶酚胺共定位的NPY在应激反应期间也会释放，且其释放与循环NA的变化比与AD的变化更密切相关。此外，应激诱导的循环NPY样免疫反应性变化并非源于肾上腺，而是主要源于周围神经系统，且NPY的释放取决于刺激的性质。
