Department of Biology, University of Padova, Padova, Italy.
Botanical Garden, University of Padova, Padova, Italy.
Methods Mol Biol. 2021;2200:167-185. doi: 10.1007/978-1-0716-0880-7_8.
Cell suspension cultures represent a widely used experimental tool suitable to perform a variety of structural and physiological studies in a more simplified system compared to the organism in toto. In this chapter we describe the methods routinely used in our laboratory to establish and maintain Arabidopsis photosynthetic and heterotrophic cell suspension cultures, containing either chloroplasts or amyloplasts, respectively. The use of these in vitro systems may allow to obtain insights into the unique features of chloroplasts versus non-green plastids, as well as their integration in the structural and metabolic compartmentalization of the plant cell.
细胞悬浮培养是一种广泛使用的实验工具，与整个生物体相比，它更适合在一个简化的系统中进行各种结构和生理研究。在本章中，我们描述了我们实验室中常规使用的方法，用于建立和维持含有叶绿体或淀粉体的拟南芥光合和异养细胞悬浮培养物。这些体外系统的使用可以帮助我们深入了解叶绿体与非绿色质体的独特特征，以及它们在植物细胞结构和代谢区室化中的整合。
