Cortese Enrico, Settimi Alessio G, Pettenuzzo Silvia, Cappellin Luca, Galenda Alessandro, Famengo Alessia, Dabalà Manuele, Antoni Vanni, Navazio Lorella
Department of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.
Department of Industrial Engineering, University of Padova, Via F. Marzolo 9, 35131 Padova, Italy.
Plants (Basel). 2021 Nov 19;10(11):2516. doi: 10.3390/plants10112516.
Increasing evidence indicates that water activated by plasma discharge, termed as plasma-activated water (PAW), can promote plant growth and enhance plant defence responses. Nevertheless, the signalling pathways activated in plants in response to PAW are still largely unknown. In this work, we analysed the potential involvement of calcium as an intracellular messenger in the transduction of PAW by plants. To this aim, (Arabidopsis) seedlings stably expressing the bioluminescent Ca reporter aequorin in the cytosol were challenged with PAW generated by a plasma torch. Ca measurement assays demonstrated the induction by PAW of rapid and sustained cytosolic Ca elevations in Arabidopsis seedlings. The dynamics of the recorded Ca signals were found to depend upon different parameters, such as the operational conditions of the torch, PAW storage, and dilution. The separate administration of nitrate, nitrite, and hydrogen peroxide at the same doses as those measured in the PAW did not trigger any detectable Ca changes, suggesting that the unique mixture of different reactive chemical species contained in the PAW is responsible for the specific Ca signatures. Unveiling the signalling mechanisms underlying plant perception of PAW may allow to finely tune its generation for applications in agriculture, with potential advantages in the perspective of a more sustainable agriculture.
越来越多的证据表明，经等离子体放电活化的水，即等离子体活化水（PAW），可以促进植物生长并增强植物的防御反应。然而，植物响应PAW而激活的信号通路仍 largely unknown。在这项工作中，我们分析了钙作为细胞内信使在植物对PAW转导中的潜在作用。为此，用等离子体火炬产生的PAW对在细胞质中稳定表达生物发光钙报告蛋白水母发光蛋白的拟南芥幼苗进行了挑战。钙测量分析表明，PAW可诱导拟南芥幼苗细胞质钙快速持续升高。发现记录的钙信号动态取决于不同参数，如火炬的操作条件、PAW的储存和稀释。以与PAW中测量的相同剂量分别施用硝酸盐、亚硝酸盐和过氧化氢不会引发任何可检测到的钙变化，这表明PAW中所含不同活性化学物质的独特混合物是特定钙信号的原因。揭示植物感知PAW的信号机制可能有助于精细调节其产生以用于农业应用，从更可持续农业的角度来看具有潜在优势。
