Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria.
Methods Mol Biol. 2021;2200:393-411. doi: 10.1007/978-1-0716-0880-7_19.
Methodological advances in coupled-mass spectrometry (gas chromatography and liquid chromatography; GC-MS and LC-MS) have rendered the profiling of highly complex plant extracts relatively facile and allowed that their high-throughput use aids the investigation of a range of biological questions. Among these is the elucidation of the genetic factors underlying metabolite abundance. For this purpose genome-wide association studies (GWAS) are being widely adopted in Arabidopsis with the resultant quantitative trait loci being subjected to cross-validation by the use of recombinant inbred lines, introgression lines, and T-DNA insertional knockout lines.
方法学的进步使得耦合质谱（气相色谱和液相色谱；GC-MS 和 LC-MS）能够相对容易地对高度复杂的植物提取物进行分析，并且允许高通量使用这些提取物来帮助研究一系列生物学问题。其中包括阐明代谢物丰度的遗传因素。为此，在拟南芥中广泛采用全基因组关联研究（GWAS），通过使用重组自交系、导入系和 T-DNA 插入敲除系对由此产生的数量性状位点进行交叉验证。
