Department of Chemical Sciences (Biochemistry Unit), Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria.
Department of Drug Sciences, University of Pavia, Pavia, Italy.
Nat Prod Res. 2022 Apr;36(8):2171-2176. doi: 10.1080/14786419.2020.1845672. Epub 2020 Nov 12.
The phytochemical constituents in the aqueous methanolic leaf extract of responsible for its nephroprotective potentials against ethane-1,2-diol induced nephrolithiasis as previously investigated in our laboratory were elucidated. The extract was prepared using 80% aqueous methanol in 72 h, Phytochemical contents of aqueous methanolic extract of (TGME) was identified using both a Thermo Scientific DSQII single quadrupole gas chromatography (GC) and a Thermo Scientific liquid chromatography (LCQ Fleet system) tandem mass spectroscopy. The chromatogram acquisition, detection of mass spectral peaks and their waveform processing were performed using Xcalibur MS Software (Thermo Scientific Inc.). GC-MS analysis revealed the presence of phenols, fatty acids, vitamins and steroids. Likewise, for LC-MS analysis kaempferol and dihydrovomifoliol-O-glucoside were detected. The identified constituents have possible contributively effect on the acclaimed pharmacological potential of against ethane-1,2-diol induced nephrolithiasis.
先前在我们实验室研究中，已阐明了负责其对乙烷-1,2-二醇诱导的肾石症的肾保护潜力的水甲醇叶提取物中的植物化学成分。使用 80%的水甲醇在 72 小时内制备提取物。使用 Thermo Scientific DSQII 单四极杆气相色谱（GC）和 Thermo Scientific 液相色谱（LCQ Fleet 系统）串联质谱法鉴定水甲醇提取物（TGME）的植物化学成分。使用 Xcalibur MS 软件（Thermo Scientific Inc.）进行色谱图采集、质谱峰检测和波形处理。GC-MS 分析表明存在酚类、脂肪酸、维生素和类固醇。同样，对于 LC-MS 分析，检测到山奈酚和二氢沃玛啡醇-O-葡萄糖苷。鉴定出的成分可能对 被认可的抗乙烷-1,2-二醇诱导的肾石症的药理学潜力有贡献作用。
