Plant Production Department, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.
Chemistry Department, Faculty of Basic Sciences and Engineering, Gonbad Kavous University, Gonbad Kavous, Iran.
J Mass Spectrom. 2022 Sep;57(9):e4884. doi: 10.1002/jms.4884.
The purpose of this study was to identify and characterize the chemical composition present in aerial parts of Prosopis farcta in petroleum ether and hydro-methanol extracts through LC-PDA-ESI-MS/MS and GC-MS techniques respectively for the first time. The plant samples were collected from northeast of Iran during maturity stage. LC-MS/MS profile revealed 47 phenolic compounds in hydro-methanol extracts, including phenolic acids, flavonoids, and their glycoside derivatives. Flavonoid-O-glycosides (19), flavonoid aglycones (11), phenolic acid derivatives (9), flavonoid-C-glycosides (4), and flavonoid-O, C-glycosides (1) were dominant class phenolics in all studied parts. The extracts contained a significant amount of major compounds, including gallic and vanillic acids, luteolin, apigenin, phloridzin, and vicenin-2. Also, GC-MS analysis of petroleum ether extracts showed that fatty acids, organic acids, steroids, terpenoids, and hydrocarbons were the group of major compounds in all parts. Twenty-four, 27, and 25 components were identified, which represent 99.2%, 96.1%, and 99.4% of the total composition in fruits, leaves, and stems, respectively. These results suggested that other genetic resources of P. farcta can be further explored to screen genotypes with high bioactive compounds and purification of phytochemical compounds, which are valuable to produce, expand, and develop natural antioxidants in production of bio-medicine and food.
本研究旨在首次通过 LC-PDA-ESI-MS/MS 和 GC-MS 技术分别鉴定和表征在石油醚和水-甲醇提取物中存在的 Prosopis farcta 地上部分的化学成分。植物样品是在伊朗东北部的成熟阶段采集的。LC-MS/MS 图谱显示水-甲醇提取物中含有 47 种酚类化合物，包括酚酸、类黄酮及其糖苷衍生物。类黄酮-O-糖苷（19 种）、类黄酮苷元（11 种）、酚酸衍生物（9 种）、类黄酮-C-糖苷（4 种）和类黄酮-O，C-糖苷（1 种）是所有研究部位中主要的酚类化合物。提取物中含有大量的主要化合物，包括没食子酸和香草酸、木樨草素、芹菜素、根皮苷和 vicenin-2。此外，石油醚提取物的 GC-MS 分析表明，脂肪酸、有机酸、甾体、萜类化合物和烃类是所有部位的主要化合物群。在果实、叶片和茎中分别鉴定出 24、27 和 25 个成分，分别代表总成分的 99.2%、96.1%和 99.4%。这些结果表明，可以进一步探索 P. farcta 的其他遗传资源，以筛选具有高生物活性化合物的基因型，并对植物化学化合物进行纯化，这对于生产、扩大和开发天然抗氧化剂在生物医学和食品生产中具有重要价值。
