Adebayo-Tayo Bukola Christianah, Ekundayo-Obaba Oluwadara, Falodun Olutayo Israel
Ibadan University, Department of Microbiology, Ibadan, Oyo State, Nigeria.
Turk J Pharm Sci. 2020 Oct;17(5):511-522. doi: 10.4274/tjps.galenos.2019.46548. Epub 2020 Oct 30.
The synthesis of nanoparticles using microorganisms and their metabolites is of increasing interest because they are potential producers of biocompatible and environmental friendly nanoparticles. Their nanoparticles can serve as potent alternatives to antibiotics against multidrug resistant (MDR) bacteria. The antibacterial potential of spp. metabolites, their silver nanoparticles (SNPs), and some antibiotics against MDR spp. was evaluated.
The antimicrobial potential of metabolites and SNPs biosynthesized from spp. was characterized, the effect of physicochemical parameters on SNP biosynthesis, the antimicrobial activity of the SNPs, and combination of SNPs and antibiotics against MDR strains were evaluated.
The bioactive metabolites of the spp. exhibited varied antimicrobial potential against the tested MDR Salmonella spp. The metabolites were able to bioreduce silver nitrate (AgNO) to Ag+ for SNP biosynthesis. Change in color from whitish to darkish brown and a surface plasma resonance peak of 600-800 nm were observed. The SNPs were aggregated, rods, and crystalline in shape and their sizes were 15 μm, 16 μm, and 13 μm. Carboxylic acid, amino acid, alcohol, esters, and aldehydes were the functional groups found in the biosynthesized SNPs. The antibacterial activity of BAC1-SNPs, BAC7-SNPs, and BAC20-SNPs against MDR Staphylococcus aureus 9 (MDRSA9) and MDRSA18 was 6.0-22 mm and 11-20 mm. SNPs biosynthesized at pH 7 and 10 mM AgNO had the highest antagonistic activity. Combination of SNPs and antibiotics exhibited the best antagonistic potential.
The metabolites and SNPs from spp. exhibited antagonistic effects against MDR spp. The combined SNPs and antibiotics had better antimicrobial activity.
利用微生物及其代谢产物合成纳米颗粒越来越受到关注，因为它们有可能产生生物相容性好且环境友好的纳米颗粒。它们的纳米颗粒可作为对抗多重耐药（MDR）细菌的抗生素的有力替代品。评估了 spp. 代谢产物、其银纳米颗粒（SNP）以及一些抗生素对 MDR spp. 的抗菌潜力。
对从 spp. 生物合成的代谢产物和 SNP 的抗菌潜力进行了表征，评估了物理化学参数对 SNP 生物合成的影响、SNP 的抗菌活性以及 SNP 与抗生素对 MDR 菌株的联合作用。
spp. 的生物活性代谢产物对测试的 MDR 沙门氏菌属表现出不同的抗菌潜力。这些代谢产物能够将硝酸银（AgNO）生物还原为 Ag+ 以进行 SNP 生物合成。观察到颜色从白色变为深棕色，表面等离子体共振峰在 600 - 800 nm。SNP 呈聚集状、棒状且为晶体，其尺寸分别为 15 μm、16 μm 和 13 μm。羧酸、氨基酸、醇、酯和醛是生物合成的 SNP 中发现的官能团。BAC1 - SNP、BAC7 - SNP 和 BAC20 - SNP 对 MDR 金黄色葡萄球菌 9（MDRSA9）和 MDRSA18 的抗菌活性为 6.0 - 22 mm 和 11 - 20 mm。在 pH 7 和 10 mM AgNO 条件下生物合成的 SNP 具有最高的拮抗活性。SNP 与抗生素的联合表现出最佳的拮抗潜力。
spp. 的代谢产物和 SNP 对 MDR spp. 表现出拮抗作用。SNP 与抗生素联合具有更好的抗菌活性。
