Samimi A S, Aghamiri S M, Babaei H, Heidarabadypor R
Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Graduated from Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Iran J Vet Res. 2020 Summer;21(3):226-229.
Acute-phase proteins (APPs) may be increased due to different stresses during estrus phase in farm animals.
Determining changes of APPs at different phases of non-synchronized estrous cycle group (NSEG), and Ovsynch-synchronized estrous cycle group (SEG) in Holstein cows.
Twelve non-pregnant clinically and paraclinicaly healthy Holstein cows with a body condition score (BCS) of 2.75 and 70 days in milk were chosen. Two groups including NSEG and SEG were performed. Blood sampling was carried out from NSEG at the time of diestrus, proestrus, and estrus. In SEG, blood sampling was performed on day 7 (at the time of prostaglandin injection, equivalent diestrus), day 9 (at the time of gonadotropin-releasing hormone (GnRH) injection, equivalent proestrus), and day 10 (at the time of insemination, equivalent estrus) of synchronization protocol. Concentrations of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), and fibrinogen (Fib) were measured.
Concentration of Hp at estrus phase was significantly higher compared with diestrus (P=0.001) and proestrus (P=0.019) in NSEG. Moreover, Hp level in the NSEG was significantly higher than SEG at estrus phase (P=0.002). Concentrations of SAA, Cp, and Fib had no significant differences during various phases of estrous cycle in each group or between equivalent phases of both groups.
It seems that unlike SAA, Fib, and Cp, concentrations of Hp may be affected by different phases of estrous cycle. Although APPs are not specific indicators, their changes besides other clinical and paraclinical indices may be helpful for more accurate heat detection in dairy cows.
家畜发情期不同应激可能导致急性期蛋白（APPs）增加。
确定荷斯坦奶牛非同步发情周期组（NSEG）和同期发情周期组（SEG）不同阶段APPs的变化。
选择12头非妊娠、临床和亚临床健康的荷斯坦奶牛，体况评分（BCS）为2.75，产奶70天。分为NSEG和SEG两组。在NSEG的间情期、发情前期和发情期进行采血。在SEG中，在同期发情方案的第7天（注射前列腺素时，相当于间情期）、第9天（注射促性腺激素释放激素（GnRH）时，相当于发情前期）和第10天（输精时，相当于发情期）进行采血。测定触珠蛋白（Hp）、血清淀粉样蛋白A（SAA）、铜蓝蛋白（Cp）和纤维蛋白原（Fib）的浓度。
NSEG中，发情期Hp浓度显著高于间情期（P = 0.001）和发情前期（P = 0.019）。此外，发情期NSEG中的Hp水平显著高于SEG（P = 0.002）。每组发情周期各阶段或两组同期阶段SAA、Cp和Fib的浓度无显著差异。
似乎与SAA、Fib和Cp不同，Hp浓度可能受发情周期不同阶段的影响。虽然APPs不是特异性指标，但它们与其他临床和亚临床指标的变化可能有助于更准确地检测奶牛发情。
