Ulutas Pinar Alkim, Musal Bayazit, Kiral Funda, Bildik Aysegul
Department of Biochemistry, Faculty of Veterinary Medicine, University of Adnan Menderes, PK 17, 09016 Aydin, Turkey.
Res Vet Sci. 2009 Jun;86(3):373-6. doi: 10.1016/j.rvsc.2008.09.001. Epub 2008 Nov 7.
The purpose of this study was to evaluate the acute phase protein (serum amyloid A, C-reactive protein, haptoglobin, ceruloplasmin and fibrinogen) profiles in pregnant dogs as well as bitches at various stages of the estrus cycle and to verify possible relationship(s) among the APPs profile and hormonal status. A total of 60 bitches of various breeds were enrolled. The animals were divided into six groups. B-mode ultrasonographic scanner (concept M/C) was used for pregnancy diagnosis, involving the first and second halves of pregnancy. Proestrus, estrus, diestrus and anestrus stages of sexual cycles were differentiated by use of vaginal cytology and blood progesterone and estradiol 17-beta levels. Serum C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin, ceruloplasmin and fibrinogen levels were determined. No statistically significant differences were observed between the groups with regard to the serum SAA and CRP concentrations. Haptoglobin, ceruloplasmin and fibrinogen concentrations were elevated in pregnant dogs compared to non-pregnant groups', independently from the pregnancy stage. No statistically significant differences in acute phase protein concentrations in dogs at various stages of estrus could be detected. Results of this study indicated that ceruloplasmin and fibrinogen levels may have potential importance in the diagnosis of pregnancy, and acute phase proteins may be used for monitoring the health status of the pregnant dogs.
本研究的目的是评估妊娠母犬以及处于发情周期不同阶段的母犬的急性期蛋白（血清淀粉样蛋白A、C反应蛋白、触珠蛋白、铜蓝蛋白和纤维蛋白原）谱，并验证急性期蛋白谱与激素状态之间可能存在的关系。总共纳入了60只不同品种的母犬。将这些动物分为六组。使用B型超声扫描仪（concept M/C）进行妊娠诊断，涵盖妊娠的前半期和后半期。通过阴道细胞学检查以及血液孕酮和雌二醇17-β水平来区分性周期的发情前期、发情期、发情后期和乏情期。测定血清C反应蛋白（CRP）、血清淀粉样蛋白A（SAA）、触珠蛋白、铜蓝蛋白和纤维蛋白原水平。在血清SAA和CRP浓度方面，各组之间未观察到统计学上的显著差异。与未妊娠组相比，妊娠母犬的触珠蛋白、铜蓝蛋白和纤维蛋白原浓度升高，且与妊娠阶段无关。在发情不同阶段的犬中，未检测到急性期蛋白浓度有统计学上的显著差异。本研究结果表明，铜蓝蛋白和纤维蛋白原水平在妊娠诊断中可能具有潜在重要性，急性期蛋白可用于监测妊娠母犬的健康状况。
