Wang Yueling, Jin Yan, Bai Yuanyuan, Song Zhen, Chu Wenjun, Zhao Mengqi, Hao Yingying, Lu Zhiming
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China.
Department of Clinical Laboratory, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China.
Exp Ther Med. 2020 Dec;20(6):235. doi: 10.3892/etm.2020.9365. Epub 2020 Oct 16.
Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using positive blood cultures (BCs) is a revolution in identification of microorganisms in clinical microbiology laboratories. Although there are several commercial pretreatment protocols they are expensive. Here, we evaluated the performance of a locally produced Bioyong pre-treatment kit for the direct identification of microorganisms in positive BCs by MALDI-TOF MS method. The mocked positive BCs were performed using 200 Thermo aerobic blood culture bottles and 200 aerobic Scenker blood culture bottles. A total of 200 organisms were invovled, including 91 strains of Gram-positive bacteria, 97 strains of Gram-negative bacteria and 12 strains of . The positive BCs were subcultured and identified by classical biochemical Vitek II testing as the gold standard of identification. The Bioyong pre-treatment kit could successfully identify microorganisms in 189 (94.5%) Thermo positive BCs and 189 (94.5%) Scenker positive blood cultures, respectively. In total, 94 (96.9%) Gram-negative bacteria, 86 (94.5%) Gram-positive bacteria and 9 (75.0%) candida isolated from Thermo positive BCs were correctly identified to species level and 95 (97.9%) Gram-negative bacteria, 86 (94.5%) Gram-positive bacteria and 8 (66.7%) candida isolated from Scenker positive BCs were correctly identified to species level. This method provides a rapid, accurate identification of bacteria and within one hour in positive blood cultures. Routine application of this technique will improve the antimicrobial treatment within 24 h among the patients with bacteremia and candidemia.
使用阳性血培养物进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析在临床微生物实验室微生物鉴定方面是一项变革。尽管有几种商业预处理方案，但它们价格昂贵。在此，我们评估了一种本地生产的Bioyong预处理试剂盒通过MALDI-TOF MS方法直接鉴定阳性血培养物中微生物的性能。使用200个Thermo需氧血培养瓶和200个Scenker需氧血培养瓶制备模拟阳性血培养物。共涉及200种微生物，包括91株革兰氏阳性菌、97株革兰氏阴性菌和12株念珠菌。将阳性血培养物进行传代培养，并通过经典生化Vitek II检测进行鉴定，作为鉴定的金标准。Bioyong预处理试剂盒分别能成功鉴定189份（94.5%）Thermo阳性血培养物和189份（94.5%）Scenker阳性血培养物中的微生物。从Thermo阳性血培养物中分离出的94株（96.9%）革兰氏阴性菌、86株（94.5%）革兰氏阳性菌和9株（75.0%）念珠菌以及从Scenker阳性血培养物中分离出的95株（97.9%）革兰氏阴性菌、86株（94.5%）革兰氏阳性菌和8株（66.7%）念珠菌均被正确鉴定到种水平。该方法能在一小时内对阳性血培养物中的细菌和念珠菌进行快速、准确的鉴定。这项技术的常规应用将改善菌血症和念珠菌血症患者在24小时内的抗菌治疗。
