Ueno Rumi, Takaoka Yuri, Shimojo Naoshi, Ohno Fumiaki, Yamaguchi Tomohiro, Matsunaga Kayoko, Kameda Makoto
Department of Pediatrics, Osaka Habikino Medical Center, Osaka, Japan.
General Research and Development Institute, Hoyu Co., Ltd., Aichi, Japan.
Asia Pac Allergy. 2020 Oct 5;10(4):e35. doi: 10.5415/apallergy.2020.10.e35. eCollection 2020 Oct.
Fish collagen is one of the major allergens involved in fish allergies; however, it has not been well-established whether fish collagen can cause anaphylaxis among individuals with fish allergies, especially children. A 9-year-old girl experienced anaphylaxis after consuming 2 fruit-flavored gummy tablets. Contacting the manufacturing company revealed that fish-derived collagen was used in the gummy tablets, and an oral fish collagen challenge test confirmed that the anaphylaxis was induced by fish collagen. Immunoblot analysis showed a 120-kDa band in a sample of the fish collagen in the gummy tablets, and no reactive band of the pork gelatin was observed. Additionally, salmon collagen and other fish samples that provoked symptoms were detected as a 120-kDa band in the immunoblot analysis. The 120-kDa band was identified as fish collagen by mass spectrometry confirming that it was the causative antigen of our patient's allergy. This case study confirms that fish collagen can cause anaphylaxis in children with fish allergies. Physicians and allergic individuals need to be aware that processed foods may contain fish collagen to avoid allergic reactions.
鱼胶原蛋白是鱼类过敏所涉及的主要过敏原之一；然而，鱼胶原蛋白是否会在鱼类过敏个体尤其是儿童中引发过敏反应，目前尚未有确凿定论。一名9岁女孩在食用2片水果味软糖后出现过敏反应。与生产公司联系后发现，软糖片中使用了鱼源胶原蛋白，口服鱼胶原蛋白激发试验证实该过敏反应是由鱼胶原蛋白引起的。免疫印迹分析显示，软糖片中的鱼胶原蛋白样品中有一条120 kDa的条带，未观察到猪肉明胶的反应性条带。此外，在免疫印迹分析中，鲑鱼胶原蛋白和其他引发症状的鱼类样品也被检测为一条120 kDa的条带。通过质谱鉴定，这条120 kDa的条带为鱼胶原蛋白，证实它是该患者过敏反应的致病抗原。本病例研究证实，鱼胶原蛋白可在鱼类过敏儿童中引发过敏反应。医生和过敏个体需要意识到加工食品可能含有鱼胶原蛋白，以避免过敏反应。
