Kim Hwan D, Lee Eunjee A, Choi Young Hwan, An Young Hyeon, Koh Rachel H, Kim Seunghyun L, Hwang Nathaniel S
School of Chemical and Biological Engineering, Institute for Chemical Processes, NBio Institute, Seoul National University, Republic of Korea.
School of Chemical and Biological Engineering, Institute for Chemical Processes, NBio Institute, Seoul National University, Republic of Korea.
Acta Biomater. 2016 Apr 1;34:21-29. doi: 10.1016/j.actbio.2016.02.022. Epub 2016 Feb 13.
Stem cells have unique ability to undergo self-renewal indefinitely in culture and potential to differentiate into almost all cell types in the human body. However, the developing a method for efficiently differentiating or manipulating these stem cells for therapeutic purposes remains a challenging problem. Pluripotent stem cells, as well as adult stem cells, require biological cues for their proliferation and differentiation. These cues are largely controlled by cell-cell, cell-insoluble factors (such as extracellular matrix), and cell-soluble factors (such as cytokine or growth factors) interactions. In this review, we describe a state of research on various stem cell-based tissue engineering applications and high throughput strategies for developing synthetic or biosynthetic microenvironments to allow efficient commitments in stem cells.
Nowadays, pluripotency of stem cells have received much attention to use therapeutic purpose. However, a major difficulty with stem cell therapy is to control its differentiation through desired cells or tissues. In other words, various microenvironment factors are involved during stem cell differentiation, including dimensionality, growth factors, cell junctions, nutritional status, matrix stiffness, matrix composition, mechanical stress, and cell-matrix adhesion. Therefore, researchers have engineered a variety of platforms to enable controlling and monitoring bioactive factors to induce stem cell commitment. In this review, we report on recent advancements in a novel technology based on high-throughput strategies for stem cell-based tissue engineering applications.
干细胞具有在培养中无限自我更新的独特能力，并有可能分化为人体几乎所有的细胞类型。然而，开发一种有效分化或操纵这些干细胞以用于治疗目的的方法仍然是一个具有挑战性的问题。多能干细胞以及成体干细胞的增殖和分化需要生物信号。这些信号在很大程度上受细胞间、细胞不溶性因子（如细胞外基质）和细胞可溶性因子（如细胞因子或生长因子）相互作用的控制。在本综述中，我们描述了基于各种干细胞的组织工程应用的研究现状以及用于开发合成或生物合成微环境以实现干细胞高效定向分化的高通量策略。
如今，干细胞的多能性在治疗用途方面备受关注。然而，干细胞治疗的一个主要困难是通过所需的细胞或组织来控制其分化。换句话说，干细胞分化过程涉及多种微环境因素，包括维度、生长因子、细胞连接、营养状态、基质硬度、基质组成、机械应力和细胞 - 基质粘附。因此，研究人员设计了各种平台来控制和监测生物活性因子以诱导干细胞定向分化。在本综述中，我们报告了基于高通量策略的用于基于干细胞的组织工程应用的新技术的最新进展。
