Department of Food Science and Technology, Graduate School of Chonnam National University, Gwangju, Korea.
Alson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, Winchester, VA, USA.
J Food Biochem. 2021 Jan;45(1):e13561. doi: 10.1111/jfbc.13561. Epub 2020 Nov 11.
Green tea contains bioactive compounds, such as polyphenols, responsible for its health-promoting effects, including antiobesity and antidiabetic effects. We previously reported that ultra-sonication extraction (UE) could efficiently increase the extraction yield of green tea compounds. In the present study, we found that the extract obtained using UE contained higher phenolic and flavonoid contents than that obtained using the conventional method. We therefore considered the extract as a bioactive metabolite-rich functional green tea extract (BMF-GTE), and tested its glucose-lowering effect by generating an adipocyte cell line stably expressing 7myc-GLUT4-GFP. We found that BMF-GTE treatment increased GLUT4 translocation to the plasma membrane. Moreover, BMF-GTE administration attenuated weight gain in mice fed a high-fat diet (HFD). Importantly, HFD-induced glucose tolerance was ameliorated in the mice receiving BMF-GTE. Therefore, we conclude that BMF-GTE worked against obesity and diabetes, at least partially, by enhancing GLUT4 translocation in adipocytes. PRACTICAL APPLICATIONS: As green tea is one of the most consumed beverages worldwide, its health effects have been widely tested. In our previous studies, we found that ultra-sonication extraction (UE) has the potential to increase the aqueous extraction yield of green tea compounds compared to conventional extraction techniques. In this study, we examined the biological effect of bioactive metabolite-rich functional green tea extract (BMF-GTE) obtained using UE; we observed that administering BMF-GTE lowered the body weight and increased insulin sensitivity in mice fed a high-fat diet, potentially by facilitating the membrane translocation of GLUT4 in adipocytes. Therefore, this study suggests that the extract obtained with UE had antiobesity and antidiabetic properties, indicative of a potential application of UE in maximizing the beneficial effects of green tea on human health.
绿茶含有生物活性化合物，如多酚，具有促进健康的作用，包括抗肥胖和抗糖尿病作用。我们之前报道过，超声提取（UE）可以有效地提高绿茶化合物的提取产率。在本研究中，我们发现 UE 提取的提取物比常规方法获得的提取物含有更高的酚类和类黄酮含量。因此，我们将提取物视为富含生物活性代谢物的功能性绿茶提取物（BMF-GTE），并通过生成稳定表达 7myc-GLUT4-GFP 的脂肪细胞系来测试其降低血糖的效果。我们发现 BMF-GTE 处理可增加 GLUT4 向质膜的易位。此外，BMF-GTE 给药可减轻高脂肪饮食（HFD）喂养小鼠的体重增加。重要的是，接受 BMF-GTE 治疗的小鼠的 HFD 诱导的葡萄糖耐量得到改善。因此，我们得出结论，BMF-GTE 通过增强脂肪细胞中 GLUT4 的易位来对抗肥胖和糖尿病，至少部分是这样。实际应用：由于绿茶是全球消费最多的饮料之一，因此其健康影响已得到广泛测试。在我们之前的研究中，我们发现与传统提取技术相比，超声提取（UE）具有提高绿茶化合物水提取产率的潜力。在这项研究中，我们检查了使用 UE 获得的富含生物活性代谢物的功能性绿茶提取物（BMF-GTE）的生物学效应；我们观察到，在高脂肪饮食喂养的小鼠中，给予 BMF-GTE 可降低体重并提高胰岛素敏感性，这可能是通过促进 GLUT4 在脂肪细胞中的膜易位来实现的。因此，这项研究表明，用 UE 提取的提取物具有抗肥胖和抗糖尿病作用，表明 UE 在最大限度地发挥绿茶对人类健康的有益作用方面具有潜在的应用。
