RJunBase：一个包含人类正常组织和癌变组织中 RNA 剪接接头的数据库。
RJunBase: a database of RNA splice junctions in human normal and cancerous tissues.
机构信息
Department of Integrative Oncology, Fudan University Shanghai Cancer Center, and the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
出版信息
Nucleic Acids Res. 2021 Jan 8;49(D1):D201-D211. doi: 10.1093/nar/gkaa1056.
Splicing is an essential step of RNA processing for multi-exon genes, in which introns are removed from a precursor RNA, thereby producing mature RNAs containing splice junctions. Here, we develope the RJunBase (www.RJunBase.org), a web-accessible database of three types of RNA splice junctions (linear, back-splice, and fusion junctions) that are derived from RNA-seq data of non-cancerous and cancerous tissues. The RJunBase aims to integrate and characterize all RNA splice junctions of both healthy or pathological human cells and tissues. This new database facilitates the visualization of the gene-level splicing pattern and the junction-level expression profile, as well as the demonstration of unannotated and tumor-specific junctions. The first release of RJunBase contains 682 017 linear junctions, 225 949 back-splice junctions and 34 733 fusion junctions across 18 084 non-cancerous and 11 540 cancerous samples. RJunBase can aid researchers in discovering new splicing-associated targets and provide insights into the identification and assessment of potential neoepitopes for cancer treatment.
剪接是多外显子基因 RNA 加工的一个重要步骤，其中内含子从前体 RNA 中被切除，从而产生含有剪接接头的成熟 RNA。在这里，我们开发了 RJunBase（www.RJunBase.org），这是一个可通过网络访问的数据库，包含三种类型的 RNA 剪接接头（线性、回文和融合接头），这些接头是从非癌和癌组织的 RNA-seq 数据中推导出来的。RJunBase 的目的是整合和描述健康或病理人类细胞和组织中所有的 RNA 剪接接头。这个新数据库促进了基因水平剪接模式和接头水平表达谱的可视化，以及未注释和肿瘤特异性接头的展示。RJunBase 的第一个版本包含了 18084 个非癌和 11540 个癌样本中 682017 个线性接头、225949 个回文接头和 34733 个融合接头。RJunBase 可以帮助研究人员发现新的与剪接相关的靶点，并为识别和评估癌症治疗的潜在新抗原提供见解。
Zotero 插件