Department of Physical and Environmental Sciences, Texas A&M University, Corpus Christi, Texas, USA.
Department of Life Sciences, Texas A&M University, Corpus Christi, Texas, USA.
J Med Food. 2021 Jul;24(7):732-740. doi: 10.1089/jmf.2020.0078. Epub 2020 Nov 11.
Diabetes Mellitus is associated with systemic inflammation and oxidative stress, which may play a central role in the development of diabetic complications. In this study, combined preparations of and metformin were investigated to determine the effects on inflammatory activity in human skeletal muscle myoblasts (HSMMs) and human diabetic skeletal muscle myoblasts (DHSMMs). Results showed that combinatorial preparations sustained cell viability for 3 days in both HSMM and DHSMM cells. However, a significant decrease in cellular viability occurred for both cell lines on day 5. Results also indicate that combinatorial preparations of may modulate immune responses by significantly upregulating proinflammatory markers, interleukin (IL) 2, and tumor necrosis factor-alpha, and upregulating the anti-inflammatory marker, IL-10, in HSMM and DHSMM cells. The combined preparations significantly downregulated the anti-inflammatory glycoprotein IL-6 in both diabetic and nondiabetic human skeletal muscle cells. The findings suggest that combined preparations of and metformin might be a potential immune-modulating agent that may promote inflammation and adversely affect the outcome of diabetic patients.
糖尿病与全身炎症和氧化应激有关，这些可能在糖尿病并发症的发展中起核心作用。在这项研究中，联合使用 和二甲双胍来确定其对人骨骼肌成肌细胞（HSMM）和人糖尿病骨骼肌成肌细胞（DHSMM）中炎症活性的影响。结果表明，组合制剂在 HSMM 和 DHSMM 细胞中均能维持细胞活力 3 天。然而，第 5 天，两种细胞系的细胞活力均显著下降。结果还表明， 联合制剂可能通过显著上调促炎标志物白细胞介素（IL）2 和肿瘤坏死因子-α，以及上调抗炎标志物白细胞介素-10（IL-10），来调节 HSMM 和 DHSMM 细胞的免疫反应。该联合制剂还显著下调了两种糖尿病和非糖尿病人骨骼肌细胞中的抗炎糖蛋白白细胞介素-6（IL-6）。这些发现表明， 和二甲双胍的联合制剂可能是一种潜在的免疫调节剂，它可能会促进炎症，并对糖尿病患者的预后产生不利影响。
