Menon Nikhil, Sparks Jean, Omoruyi Felix O
Department of Life Sciences, Texas A&M University, Corpus Christi, Texas, USA.
Pharmacognosy Res. 2016 Apr-Jun;8(2):85-8. doi: 10.4103/0974-8490.172656.
Diabetes mellitus is a chronic metabolic disease that according to the World Health Organization affects more than 382 million people. The rise in diabetes mellitus coupled with the lack of an effective treatment has led many to investigate medicinal plants to identify a viable alternative.
To evaluate red blood cell (RBC) membrane adenosine triphosphatase (ATPase) activities and antioxidant levels in streptozotocin-induced diabetic rats administered aqueous preparation of Kalanchoe pinnata leaves.
Diabetes mellitus was induced in rats by a single administration of streptozotocin (60 mg/kg). Diabetic rats were then treated with aqueous K. pinnata preparation (three mature leaves ~ 9.96 g/70 kg body weight or about 0.14 g/kg body weight/day) for 30 days. Serum glucose, RBC membrane ATPase activities, and antioxidant levels were determined.
We noted weight loss and reduced food consumption in the treated diabetic group. Serum glucose levels were reduced in the treated diabetic group compared to the other groups. Superoxide dismutase activity and glutathione levels were not significantly elevated in the treated group compared to the diabetic group. However, serum catalase activity was significantly (P < 0.05) increased in the treated diabetic group compared to the other groups. Serum thiobarbituric acid reactive substances were not significantly altered among the groups. There was a significant (P < 0.05) increase in Mg(2+) ATPase activity and a nonsignificant increase in Na(+)/K(+) ATPase activity in the RBC membrane of the treated diabetic group compared to the diabetic group.
The consumption of aqueous preparation of K. pinnata may accrue benefits in the management of diabetes by lowering oxidative stress often associated with the disease and improving the availability of cellular magnesium through an increase in the magnesium ATPase pump in the RBC membrane for increased cellular metabolism of glucose through the glycolytic pathway.
We noted weight loss and reduced food consumption in the diabetic rats treated with K. pinnata preparationSerum glucose levels were reduced in diabetic rats treated with K. pinnata preparationSerum catalase activity was significantly (P < 0.05) increased in diabetic rats treated with K. pinnata preparationWe also noted a significant (P < 0.05) increase in Mg(2+) ATPase activity in the RBC membranes of diabetic rats treated with K. pinnata preparationOverall, the consumption of aqueous preparation of K. pinnata lowered oxidative stress often associated with diabetes and improved availability of cellular magnesium through an increase in magnesium ATPase pump in the RBC membrane.
糖尿病是一种慢性代谢疾病，据世界卫生组织统计，全球有超过3.82亿人受其影响。糖尿病发病率的上升以及缺乏有效的治疗方法，促使许多人研究药用植物以寻找可行的替代方案。
评估给予落地生根叶水制剂的链脲佐菌素诱导的糖尿病大鼠红细胞（RBC）膜三磷酸腺苷酶（ATPase）活性和抗氧化水平。
通过单次注射链脲佐菌素（60mg/kg）诱导大鼠患糖尿病。然后用落地生根水制剂（三片成熟叶~9.96g/70kg体重或约0.14g/kg体重/天）治疗糖尿病大鼠30天。测定血清葡萄糖、RBC膜ATPase活性和抗氧化水平。
我们注意到治疗后的糖尿病组体重减轻且食物摄入量减少。与其他组相比，治疗后的糖尿病组血清葡萄糖水平降低。与糖尿病组相比，治疗组超氧化物歧化酶活性和谷胱甘肽水平没有显著升高。然而，与其他组相比，治疗后的糖尿病组血清过氧化氢酶活性显著（P<0.05）升高。各组间血清硫代巴比妥酸反应性物质无显著变化。与糖尿病组相比，治疗后的糖尿病组RBC膜中Mg(2+)ATPase活性显著（P<0.05）升高，而Na(+)/K(+)ATPase活性无显著升高。
食用落地生根水制剂可能通过降低常与该疾病相关的氧化应激，并通过增加RBC膜中镁ATP酶泵来提高细胞内镁的可用性，从而改善葡萄糖通过糖酵解途径的细胞代谢，对糖尿病的管理有益。
我们注意到用落地生根制剂治疗的糖尿病大鼠体重减轻且食物摄入量减少用落地生根制剂治疗的糖尿病大鼠血清葡萄糖水平降低用落地生根制剂治疗的糖尿病大鼠血清过氧化氢酶活性显著（P<0.05）升高我们还注意到用落地生根制剂治疗的糖尿病大鼠RBC膜中Mg(2+)ATPase活性显著（P<0.05）升高总体而言，食用落地生根水制剂降低了常与糖尿病相关的氧化应激，并通过增加RBC膜中镁ATP酶泵提高了细胞内镁的可用性。
