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冷冻牛包皮黏液可作为弯曲菌属胎儿亚种. 生殖器直接分子诊断的合适样本。

Frozen bovine preputial mucus as a suitable sample for the direct molecular diagnosis of Campylobacter fetus subsp. venerealis.

机构信息

Laboratório de Bacteriologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil.

Laboratório de Bacteriologia Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9090, 91540-000 Porto Alegre, RS, Brazil.

出版信息

J Microbiol Methods. 2020 Dec;179:106101. doi: 10.1016/j.mimet.2020.106101. Epub 2020 Nov 9.

DOI:10.1016/j.mimet.2020.106101
PMID:33181206
Abstract

Long transportation times remain a challenge to the satisfactory diagnosis of Campylobacter fetus subsp. venerealis (Cfv). Here we demonstrated that samples of frozen bovine preputial mucus maintained at -20 °C for 10 days can be used as an alternative source for molecular diagnosis of Cfv. This approach will improve the analysis of this bacterium.

摘要

长途运输时间仍然是满足弯曲菌胎儿亚种(Cfv)诊断的挑战。在这里,我们证明了在-20°C 下保存 10 天的冷冻牛包皮粘液样本可用作 Cfv 分子诊断的替代来源。这种方法将改善对该细菌的分析。

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